一项初步研究表明，miR-31-3p 和 miR-196a-5p 作为晚期喉癌的非侵入性生物标志物具有潜力。

A pilot study reveals the potential of miR-31-3p and miR-196a-5p as non-invasive biomarkers in advanced laryngeal cancer.

机构信息

Medical University of Sofia, Sofia, Bulgaria.

出版信息

Folia Med (Plovdiv). 2021 Jun 30;63(3):355-364. doi: 10.3897/folmed.63.e55346.

DOI:10.3897/folmed.63.e55346

PMID:34196150

Abstract

INTRODUCTION

Recently, miRNAs have become popular molecules used as non-invasive biomarkers in cancer diseases.

AIM

The aim of the study was to explore the expression of four miRNAs isoforms: miR-31-3p, miR-196a-5p, miR-210-3p and miR-424-5p in plasma and tissue samples from patients with advanced laryngeal squamous cell carcinoma (LSCC) and healthy controls.

MATERIALS AND METHODS

Fresh-frozen tumour and normal laryngeal tissue as well as plasma samples were obtained from 22 patients diagnosed with advanced LSCC. The control group included plasma samples from 21 cancer-free volunteers. Total RNA (including miRNAs) extraction, reverse transcription and real time qPCR were the laboratory techniques used in the study. The obtained results were analyzed using SPSS software v. 23.

RESULTS

We found that miR-31-3p, miR-196a-5p, and miR-210-3p levels were significantly elevated in laryngeal tumour tissue, but only the levels of miR-31-3p and miR-196a-5p were significantly upregulated in the plasma LSCC target group. Positive correlation was obtained for miR-31-3p (rs=0.443, p=0.039) and miR-196a-5p (rs=0.548; p=0.008) between plasma and adjacent tumour tissue LSCC samples. ROC analyses were used to evaluate the discriminative power of both miRNAs alone and in combination. The combination of miR-31-3p and miR-196a-5p showed best results with AUC=0.978 (95% CI: 0.945-1.000, p.

摘要

简介

最近，miRNAs 已成为癌症疾病中用作非侵入性生物标志物的热门分子。

目的

本研究旨在探讨四种 miRNA 亚型：miR-31-3p、miR-196a-5p、miR-210-3p 和 miR-424-5p 在晚期喉鳞状细胞癌 (LSCC) 患者和健康对照者的血浆和组织样本中的表达。

材料和方法

从 22 名诊断为晚期 LSCC 的患者中获得新鲜冷冻的肿瘤和正常喉组织以及血浆样本。对照组包括 21 名无癌症志愿者的血浆样本。研究中使用的实验室技术包括总 RNA（包括 miRNAs）提取、逆转录和实时 qPCR。使用 SPSS 软件 v.23 分析获得的结果。

结果

我们发现 miR-31-3p、miR-196a-5p 和 miR-210-3p 在喉肿瘤组织中的水平显着升高，但只有 miR-31-3p 和 miR-196a-5p 的水平在血浆 LSCC 靶组中显着上调。miR-31-3p（rs=0.443，p=0.039）和 miR-196a-5p（rs=0.548；p=0.008）在血浆和相邻肿瘤组织 LSCC 样本之间存在正相关。ROC 分析用于评估这两种 miRNA 单独和组合的区分能力。miR-31-3p 和 miR-196a-5p 的组合具有最佳结果，AUC=0.978（95%CI：0.945-1.000，p<0.001）。