Department of Biochemistry, Faculty of Pharmacy, Trakya University, Edirne, 22030, Turkey.
J Cancer Res Clin Oncol. 2013 Sep;139(9):1499-506. doi: 10.1007/s00432-013-1469-2. Epub 2013 Jul 2.
Altered microRNA (miRNA) expression has been found in many cancers, including lung, breast, prostate, bladder and colorectal cancer. Many recent studies have demonstrated that aberrant plasma miRNAs were also found in various types of cancers. However, the alteration in plasma miRNA expressions in laryngeal squamous cell carcinoma (LSCC) remains unclear. The present study aimed to investigate the alterations in plasma miRNAs in LSCC.
In the present study, the expression profiles of 738 miRNAs in plasma from 20 patients and 44 healthy subjects were evaluated using high-throughput real-time quantitative polymerase chain reaction.
Our results demonstrated that expression levels of 17 miRNAs were significantly upregulated in patients with LSCCs when compared to control group (p < 0.05). Expression levels of nine miRNAs were found significantly downregulated in LSCC patients (p < 0.05). In addition, 17 miRNAs were expressed only in LSCC group, and five of these miRNAs (miR-331-3p, 603, 1303, 660-5p and 212-3p) are LSCC specific and never seen before in plasma of any human subject.
In conclusion, our study suggests that detecting these LSCC-specific miRNAs in plasma might serve as novel noninvasive biomarkers for LSCC.
已在多种癌症中发现微小 RNA（miRNA）表达异常，包括肺癌、乳腺癌、前列腺癌、膀胱癌和结直肠癌。许多最近的研究表明，各种类型的癌症中也存在异常的血浆 miRNA。然而，喉鳞状细胞癌（LSCC）中血浆 miRNA 表达的改变尚不清楚。本研究旨在探讨 LSCC 中血浆 miRNA 的变化。
在本研究中，使用高通量实时定量聚合酶链反应评估了 20 例患者和 44 例健康受试者血浆中 738 种 miRNA 的表达谱。
我们的结果表明，与对照组相比，LSCC 患者的 17 种 miRNA 表达水平显著上调（p<0.05）。在 LSCC 患者中发现 9 种 miRNA 的表达水平显著下调（p<0.05）。此外，17 种 miRNA 仅在 LSCC 组中表达，其中 5 种 miRNA（miR-331-3p、603、1303、660-5p 和 212-3p）是 LSCC 特异性的，以前从未在任何人类受试者的血浆中发现过。
总之，我们的研究表明，检测这些 LSCC 特异性 miRNA 在血浆中可能作为 LSCC 的新型非侵入性生物标志物。
