Feng Guodong, Zhuang Yiping, Feng Jianfang, Zhao Jiawei, Zhong Chuan, Chen Shilin, Chen Jun
Department of Radiology, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, Jiangsu, P.R. China.
Department of Radiology, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, Jiangsu, P.R. China.
J Surg Res. 2021 Nov;267:358-365. doi: 10.1016/j.jss.2021.05.038. Epub 2021 Jun 28.
The CT-guided percutaneous puncture-inoculation for establishing the rabbit VX2 lung cancer model (LCM) is time-consuming, requires repeated CT scans, and has a high complication rate. Therefore, this study aimed to develop a navigational template using 3D technology to provide an alternative method for establishing the model with improved success and complication rates.
Ideal pressure was determined using chest CT data from 15 anesthetized rabbits fitted with sphygmomanometer cuff around their chests. Subsequently, a preliminary 3D template with a square window and cross-sign to facilitate precise installation was designed. Using another 20 rabbits fixed with the preliminary template, an ideal common puncture point and parameter were determined, a navigational tunnel was set up on the template surface, and the final puncture navigational template was printed out. Eight-four rabbits (42/group) were assigned to the experimental (template-guided puncture) and control (traditional puncutre) groups and underwent VX2 tumor-fragment inoculation to validate the template. Differences in various parameters between two groups were analyzed.
The ideal pressure was 30 mmHg. All rabbits were inoculated successfully and the template adequately fit the rabbit chest. The experimental group displayed significantly better operation time (198.93±36.64 vs 735.14±91.19 seconds); number of CT scans (0 vs 7.19±1.64); pneumothorax (11.9% vs 35.7%), chest seeding (16.7% vs 35.7%), and mid-lung field tumor-bearing (88.1% vs 59.5%) rates than the control group (all, P <0.05). The groups did not differ in rib injury, tumor volume or survival time (all, P > 0.05).
We successfully developed a puncture navigational template, providing an alternative method for establishing the rabbit VX2 LCM.
CT引导下经皮穿刺接种建立兔VX2肺癌模型（LCM）耗时，需要反复进行CT扫描，且并发症发生率高。因此，本研究旨在利用三维技术开发一种导航模板，为建立该模型提供一种替代方法，以提高成功率和降低并发症发生率。
使用15只胸部佩戴血压计袖带的麻醉兔的胸部CT数据确定理想压力。随后，设计了一个带有方形窗口和十字标记以方便精确安装的初步三维模板。使用另外20只固定有初步模板的兔子，确定理想的共同穿刺点和参数，在模板表面设置导航通道，并打印出最终的穿刺导航模板。将84只兔子（每组42只）分为实验组（模板引导穿刺）和对照组（传统穿刺），进行VX2肿瘤碎片接种以验证模板。分析两组之间各种参数的差异。
理想压力为30 mmHg。所有兔子均成功接种，且模板与兔胸部贴合良好。实验组的手术时间（198.93±36.64对735.14±91.19秒）、CT扫描次数（0对7.19±1.64）、气胸发生率（11.9%对35.7%）、胸部种植率（16.7%对35.7%）和肺中叶肿瘤携带率（88.1%对59.5%）均显著优于对照组（均P<0.05）。两组在肋骨损伤、肿瘤体积或生存时间方面无差异（均P>0.05）。
我们成功开发了一种穿刺导航模板，为建立兔VX2 LCM提供了一种替代方法。
