Rosenau Simon, Oertel Elisa, Mott Alexander Charles, Tetens Jens
Department of Animal Science, Georg-August-University of Göttingen, 37077 Göttingen, Germany.
Center for Integrated Breeding Research, Georg-August-University of Göttingen, 37075 Göttingen, Germany.
Life (Basel). 2021 Jun 14;11(6):558. doi: 10.3390/life11060558.
An increasing number of fishmeal supplements are becoming the focus of aquaculture research, with a special emphasis on microalgae/cyanobacteria such as spirulina being considered as sustainable alternatives. New feed ingredients can have a far-reaching impact on the intestinal microbiome and therefore play an important role in the development and the health of fish. However, the influence of these alternatives on the microbiome is largely unknown. We undertook a 10 weeks feeding experiment on 120 African catfish with an initial body weight of 50.1 ± 2.95 g. To understand the effect of the spirulina supplementation, two isoenergetic experimental diets were formulated, containing either fishmeal or spirulina as a protein source. The 16S rRNA sequencing was used to analyze the intestinal bacteria microbiota. Results show that the observed richness indicated no significant statistical difference, but Chao1, ACE, Shannon, and Simpson indices indicate a possible increase in bacterial richness for the spirulina diet. The most abundant bacteria in both experimental groups were with the only taxa from the genus . The bacterium from genus was more likely to be found in the microbiome of fish fed the fishmeal diet. In spirulina-fed fish, the genera and were the most dominant microbes observed. Even though some genera were more abundant in the spirulina group, the overall microbial community structure was not affected by diets.
越来越多的鱼粉补充剂正成为水产养殖研究的焦点，特别强调将微藻/蓝细菌（如螺旋藻）视为可持续的替代品。新的饲料成分可能会对肠道微生物群产生深远影响，因此在鱼类的发育和健康中发挥重要作用。然而，这些替代品对微生物群的影响在很大程度上尚不清楚。我们对120条初始体重为50.1±2.95克的非洲鲶鱼进行了为期10周的喂养实验。为了解添加螺旋藻的效果，配制了两种等能量的实验饲料，分别以鱼粉或螺旋藻作为蛋白质来源。采用16S rRNA测序分析肠道细菌微生物群。结果表明，观察到的丰富度无显著统计学差异，但Chao1、ACE、Shannon和Simpson指数表明，螺旋藻饲料组的细菌丰富度可能有所增加。两个实验组中最丰富的细菌均来自唯一的属。该属细菌更可能在喂食鱼粉饲料的鱼的微生物群中发现。在喂食螺旋藻的鱼中，属和属是观察到的最主要微生物。尽管螺旋藻组中一些属更为丰富，但总体微生物群落结构不受饲料影响。
