Tesena Parichart, Kingkaw Amornthep, Vongsangnak Wanwipa, Pitikarn Surakiet, Phaonakrop Narumon, Roytrakul Sittiruk, Kovitvadhi Attawit
Graduate Student in Animal Health and Biomedical Science Program, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
Department of Clinical Science and Public Health, Faculty of Veterinary Science, Mahidol University, Salaya, Puttamonthon, Nakhon Pathom 73170, Thailand.
Animals (Basel). 2021 Jun 27;11(7):1913. doi: 10.3390/ani11071913.
Equine melanocytic neoplasm (EMN) is a cutaneous neoplasm and is mostly observed in aged grey horses. This preliminary study aimed to identify potential proteins to differentiate normal, mild and severe EMN from serum proteomic profiling. Serum samples were collected from 25 grey horses assigned to three groups: normal (free of EMN; = 10), mild ( = 6) and severe EMN ( = 9). To explore the differences in proteins between groups, proteomic profiling and analysis were employed. Accordingly, 8241 annotated proteins out of 8725 total proteins were compared between normal and EMN groups and inspected based on differentially expressed proteins (DEPs). Through DEP analysis, 95 significant DEPs differed between normal and EMN groups. Among these DEPs, 41 significant proteins were categorised according to protein functions. Based on 41 significant proteins, 10 were involved in metabolism and 31 in non-metabolism. Interestingly, phospholipid phosphatase6 (PLPP6) and ATPase subunit alpha (Na+/K+-ATPase) were considered as potential proteins uniquely expressed in mild EMN and related to lipid and energy metabolism, respectively. Non-metabolism-related proteins (BRCA1, phosphorylase B kinase regulatory subunit: PHKA1, tyrosine-protein kinase receptor: ALK and rho-associated protein kinase: ROCK1) correlated to melanoma development differed among all groups. The results of our study provide a foundation for early EMN biomonitoring and prevention.
马黑素细胞肿瘤（EMN）是一种皮肤肿瘤，多见于老年灰色马匹。这项初步研究旨在从血清蛋白质组学分析中鉴定出能够区分正常、轻度和重度EMN的潜在蛋白质。从25匹灰色马匹中采集血清样本，分为三组：正常组（无EMN；n = 10）、轻度组（n = 6）和重度EMN组（n = 9）。为了探究各组之间蛋白质的差异，采用了蛋白质组学分析方法。据此，在总共8725种蛋白质中，对8241种注释蛋白质在正常组和EMN组之间进行了比较，并基于差异表达蛋白质（DEP）进行了检查。通过DEP分析，正常组和EMN组之间有95种显著的DEP存在差异。在这些DEP中，41种显著蛋白质根据蛋白质功能进行了分类。基于这41种显著蛋白质，10种参与代谢，31种参与非代谢。有趣的是，磷脂磷酸酶6（PLPP6）和ATP酶亚基α（Na+/K+-ATP酶）被认为是分别在轻度EMN中独特表达且与脂质和能量代谢相关的潜在蛋白质。与黑色素瘤发展相关的非代谢相关蛋白质（BRCA1、磷酸化酶B激酶调节亚基：PHKA1、酪氨酸蛋白激酶受体：ALK和rho相关蛋白激酶：ROCK1）在所有组中存在差异。我们的研究结果为EMN的早期生物监测和预防提供了基础。
