泰国菌株多样性和抗生素耐药性模式的时间变化
Temporal Variations in Patterns of Strain Diversity and Antibiotic Resistance in Thailand.
作者信息
Wongkuna Supapit, Janvilisri Tavan, Phanchana Matthew, Harnvoravongchai Phurt, Aroonnual Amornrat, Aimjongjun Sathid, Malaisri Natamon, Chankhamhaengdecha Surang
机构信息
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand.
出版信息
Antibiotics (Basel). 2021 Jun 13;10(6):714. doi: 10.3390/antibiotics10060714.
has been recognized as a life-threatening pathogen that causes enteric diseases, including antibiotic-associated diarrhea and pseudomembranous colitis. The severity of infection (CDI) correlates with toxin production and antibiotic resistance of In Thailand, the data addressing ribotypes, toxigenic, and antimicrobial susceptibility profiles of this pathogen are scarce and some of these data sets are limited. In this study, two groups of isolates in Thailand, including 50 isolates collected from 2006 to 2009 (THA group) and 26 isolates collected from 2010 to 2012 (THB group), were compared for toxin genes and ribotyping profiles. The production of toxins A and B were determined on the basis of toxin gene profiles. In addition, minimum inhibitory concentration of eight antibiotics were examined for all 76 isolates. The isolates of the THA group were categorized into 27 (54%) and 23 (46%), while the THB isolates were classified into five toxigenic profiles, including six (23%), two (8%), five (19%), seven (27%), and six (23%). By visually comparing them to the references, only five ribotypes were identified among THA isolates, while 15 ribotypes were identified within THB isolates. Ribotype 017 was the most common in both groups. Interestingly, 18 unknown ribotyping patterns were identified. Among eight -positive isolates, three isolates showed significantly greater levels of toxin A than the reference strain. The levels of toxin B in 3 of 47 -positive isolates were significantly higher than that of the reference strain. Based on the antimicrobial susceptibility test, metronidazole showed potent efficiency against most isolates in both groups. However, high MIC values of cefoxitin (MICs 256 μg/mL) and chloramphenicol (MICs ≥ 64 μg/mL) were observed with most of the isolates. The other five antibiotics exhibited diverse MIC values among two groups of isolates. This work provides evidence of temporal changes in both strains and patterns of antimicrobial resistance in Thailand.
已被公认为一种危及生命的病原体，可导致肠道疾病，包括抗生素相关性腹泻和伪膜性结肠炎。艰难梭菌感染（CDI）的严重程度与毒素产生及该病原体的抗生素耐药性相关。在泰国，有关这种病原体的核糖分型、产毒素情况及抗菌药物敏感性概况的数据稀缺，且其中一些数据集有限。在本研究中，对泰国的两组分离株进行了毒素基因和核糖分型分析比较，其中一组是2006年至2009年收集的50株分离株（THA组），另一组是2010年至2012年收集的26株分离株（THB组）。根据毒素基因概况确定毒素A和B的产生情况。此外，对所有76株分离株检测了八种抗生素的最低抑菌浓度。THA组的分离株分为27种（54%）和23种（46%）产毒素类型，而THB组的分离株分为五种产毒素类型，包括六种（23%）、两种（8%）、五种（19%）、七种（27%）和六种（23%）。通过与参考菌株进行视觉比较，在THA组分离株中仅鉴定出五种核糖型，而在THB组分离株中鉴定出15种核糖型。核糖型017在两组中最为常见。有趣的是，鉴定出18种未知的核糖分型模式。在8株毒素阳性分离株中，有3株的毒素A水平显著高于参考菌株。47株毒素阳性分离株中有3株的毒素B水平显著高于参考菌株。基于抗菌药物敏感性试验，甲硝唑对两组中的大多数分离株均显示出高效。然而，大多数分离株对头孢西丁（MICs为256μg/mL）和氯霉素（MICs≥64μg/mL）的MIC值较高。其他五种抗生素在两组分离株中的MIC值各不相同。这项工作为泰国艰难梭菌菌株及其抗菌药物耐药模式的时间变化提供了证据。
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