Mortensen Ninell P, Moreno Caffaro Maria, Aravamudhan Shyam, Beeravalli Lakshmi, Prattipati Sharmista, Snyder Rodney W, Watson Scott L, Patel Purvi R, Weber Frank X, Montgomery Stephanie A, Sumner Susan J, Fennell Timothy R
RTI International, 3040 E Cornwallis Road, Research Triangle Park, NC 27709, USA.
Joint School of Nanoscience and Nanoengineering, 2907 East Gate City Blvd., Greensboro, NC 27401, USA.
Nanomaterials (Basel). 2021 Jun 4;11(6):1487. doi: 10.3390/nano11061487.
Oral exposure to nanoparticles (NPs) during early life is an understudied area. The goals of this study were to evaluate the effect of pre-weaned rat gastric fluids on 50 nm CuO NPs and TiO E171 in vitro, and to evaluate uptake in vivo. The NP uptake was studied in vivo in male and female Sprague-Dawley rat pups following oral administration of four consecutive daily doses of 10 mg/kg CuO NPs, TiO E171, or vehicle control (water) between postnatal day (PND) 7-10. Rat pups were sacrificed on either PND10 or PND21. Simulated digestion led to dissolution of CuO NPs at the later ages tested (PND14 and PND21, but not PND7). In vivo intestinal uptake of CuO NPs and TiO E171 was observed by hyperspectral imaging of intestinal cross sections. Brightfield microscopy showed that the number of immune cells increased in the intestinal tissue following NP administration. Orally administered NPs led to low intestinal uptake of NPs and an increase in immune cells in the small and large intestine, suggesting that oral exposure to NPs during early life may lead to irritation or a low-grade inflammation. The long-term impact of increased immune cells in the intestinal tract during early life is unknown.
生命早期经口暴露于纳米颗粒（NPs）是一个研究较少的领域。本研究的目的是评估断奶前大鼠胃液对50 nm氧化铜纳米颗粒（CuO NPs）和二氧化钛E171（TiO E171）的体外作用，并评估其体内摄取情况。在出生后第7至10天，对雄性和雌性斯普拉格-道利大鼠幼崽连续4天每天口服10 mg/kg的CuO NPs、TiO E171或载体对照（水），之后在体内研究纳米颗粒的摄取情况。在出生后第10天或第21天处死大鼠幼崽。模拟消化导致在测试的较晚年龄段（出生后第14天和第21天，但不是第7天）CuO NPs溶解。通过肠道横截面的高光谱成像观察CuO NPs和TiO E171在体内的肠道摄取情况。明场显微镜显示，纳米颗粒给药后肠道组织中的免疫细胞数量增加。经口给药的纳米颗粒导致纳米颗粒在肠道的低摄取以及小肠和大肠中免疫细胞增加，这表明生命早期经口暴露于纳米颗粒可能会导致刺激或低度炎症。生命早期肠道中免疫细胞增加的长期影响尚不清楚。
