Department of Immunology and Infectious Biology, Faculty of Biology and Environmental Protection, Institute of Microbiology, Biotechnology and Immunology, University of Lodz, Banacha Str. 12/16, 90-237 Lodz, Poland.
Department of Microbiology, School of Natural Sciences, National University of Ireland Galway, SW4 Galway, Ireland.
Cells. 2021 Jun 4;10(6):1385. doi: 10.3390/cells10061385.
Lipopolysaccharide (LPS) of (Hp) bacteria causes disintegration of gastric tissue cells in vitro. It has been suggested that interleukin (IL)-33 is involved in healing gastric injury.
To elucidate whether Hp LPS affects regeneration of gastric barrier initiated by IL-33.
Primary gastric epithelial cells or fibroblasts from were transfected with siRNA IL-33. Such cells, not exposed or treated with LPS Hp, were sub-cultured in the medium with or without exogenous IL-33. Then cell migration was assessed in conjunction with oxidative stress and apoptosis, activation of extracellular signal-regulated kinase (Erk), production of collagen I and soluble ST2 (IL-33 decoy).
Control cells not treated with LPS Hp migrated in the presence of IL-33. The pro-regenerative activity of IL-33 was related to stimulation of cells to collagen I production. Wound healing by cells exposed to LPS Hp was inhibited even in the presence of IL-33. This could be due to increased oxidative stress and apoptosis in conjunction with Erk activation, sST2 elevation and modulation of collagen I production.
The recovery of gastric barrier cells during Hp infection potentially can be affected due to downregulation of pro-regenerative activity of IL-33 by LPS Hp.
（Hp）细菌的脂多糖（LPS）在体外导致胃组织细胞解体。有人提出白细胞介素（IL）-33 参与胃损伤的愈合。
阐明 Hp LPS 是否会影响 IL-33 引发的胃屏障再生。
用 siRNA IL-33 转染原代胃上皮细胞或成纤维细胞。未暴露或未用 LPS Hp 处理的此类细胞在有或没有外源性 IL-33 的培养基中进行亚培养。然后评估细胞迁移以及氧化应激和细胞凋亡、细胞外信号调节激酶（Erk）的激活、胶原 I 和可溶性 ST2（IL-33 诱饵）的产生。
未用 LPS Hp 处理的对照细胞在存在 IL-33 的情况下迁移。IL-33 的促再生活性与刺激细胞产生胶原 I 有关。即使存在 IL-33，暴露于 LPS Hp 的细胞的伤口愈合也受到抑制。这可能是由于氧化应激和细胞凋亡增加，同时伴随着 Erk 激活、sST2 升高和胶原 I 产生的调节。
由于 LPS Hp 下调了 IL-33 的促再生活性，因此在 Hp 感染期间胃屏障细胞的恢复可能会受到影响。
