He Qianxian, Li Jingyu, Ma Yongkai, Chen Qi, Chen Gu
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
Microorganisms. 2021 Jun 4;9(6):1224. doi: 10.3390/microorganisms9061224.
'Chachiensis' (PCR-Chachiensis), the pericarps of Blanco cv. Chachiensis, is a food condiment and traditional medicine in southeast and eastern Asia. Its rich and various bacterial community awaits exploration. The present study is the first report on probiotic screening and characterization of bacteria from PCR-Chachiensis. Based on 64 culturable bacterial isolates, 8 strains were screened out to have great survival in the simulated gastrointestinal stressful condition, being nonhemolytic and without biogenic amine formation. They were identified by 16S rRNA gene sequencing as two , three , and three strains from Bacillales. Their probiotic properties, cholesterol-lowering potential and carbohydrate utilization capability were further investigated. Though these eight strains all displayed distinct cholesterol removal potential, N17-02 showed both remarkable cholesterol removal capability and presence of bile salt hydrolase gene, as well as possessing most of the desirable probiotic attributes. Thus, it could be a good probiotic candidate with hypocholesterolemic potential. N17-12 displayed the widest carbohydrate utilization profile and the strongest antimicrobial activity. Hence, it was promising to be used as a probiotic in a host and as a fermentation starter in fermented food or feed.
“Chachiensis”（PCR-Chachiensis），即布兰科品种Chachiensis的果皮，是东南亚和东亚地区的一种食品调味料和传统药物。其丰富多样的细菌群落有待探索。本研究是关于PCR-Chachiensis中细菌的益生菌筛选和特性鉴定的首次报告。基于64株可培养细菌分离株，筛选出8株在模拟胃肠道应激条件下具有良好存活率、无溶血现象且不产生生物胺的菌株。通过16S rRNA基因测序将它们鉴定为来自芽孢杆菌目的两株、三株和三株。进一步研究了它们的益生菌特性、降胆固醇潜力和碳水化合物利用能力。尽管这八株菌株都表现出不同的胆固醇去除潜力，但N17-02既显示出显著的胆固醇去除能力和胆盐水解酶基因的存在，又具备大多数理想的益生菌特性。因此，它可能是一种具有降胆固醇潜力的良好益生菌候选菌株。N17-12表现出最广泛的碳水化合物利用谱和最强的抗菌活性。因此，它有望作为宿主中的益生菌以及发酵食品或饲料中的发酵剂。
