IECL, Université de Lorraine, CNRS, Inria, F-54000 Nancy, France.
LCPME, Université de Lorraine, CNRS, F-54000 Nancy, France.
Viruses. 2021 Jun 4;13(6):1076. doi: 10.3390/v13061076.
The World Health Organisation recommends monitoring the circulation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We investigated anti-SARS-CoV-2 total immunoglobulin (IgT) antibody seroprevalence and in vitro sero-neutralization in Nancy, France, in spring 2020. Individuals were randomly sampled from electoral lists and invited with household members over 5 years old to be tested for anti-SARS-CoV-2 (IgT, i.e., IgA/IgG/IgM) antibodies by ELISA (Bio-rad); the sero-neutralization activity was evaluated on Vero CCL-81 cells. Among 2006 individuals, the raw seroprevalence was 2.1% (95% confidence interval 1.5 to 2.9), was highest for 20- to 34-year-old participants (4.7% (2.3 to 8.4)), within than out of socially deprived area (2.5% vs. 1%, = 0.02) and with than without intra-family infection ( < 10). Moreover, 25% of participants presented at least one COVID-19 symptom associated with SARS-CoV-2 positivity ( < 10), with highly discriminant anosmia or ageusia (odds ratio 27.8 [13.9 to 54.5]); 16.3% (6.8 to 30.7) of seropositive individuals were asymptomatic. Positive sero-neutralization was demonstrated in vitro for 31/43 seropositive subjects. Regarding the very low seroprevalence, a preventive effect of the lockdown in March 2020 can be assumed for the summer, but a second COVID-19 wave, as expected, could be subsequently observed in this poorly immunized population.
世界卫生组织建议监测严重急性呼吸系统综合症冠状病毒 2（SARS-CoV-2）的循环情况。我们在法国南锡调查了 2020 年春季的 SARS-CoV-2 总免疫球蛋白（IgT）抗体血清流行率和体外血清中和作用。从选举名单中随机抽取个体，并邀请 5 岁以上的家庭成员通过 ELISA（Bio-rad）检测抗 SARS-CoV-2（IgT，即 IgA/IgG/IgM）抗体；在 Vero CCL-81 细胞上评估血清中和活性。在 2006 名个体中，原始血清流行率为 2.1%（95%置信区间 1.5 至 2.9），20 至 34 岁参与者最高（4.7%（2.3 至 8.4）），处于社会贫困地区内（2.5%比 1%， = 0.02）和有家庭内感染的参与者比没有家庭内感染的参与者高（ < 10）。此外，25%的参与者出现至少一种与 SARS-CoV-2 阳性相关的 COVID-19 症状（ < 10），且伴有高度鉴别性的嗅觉丧失或味觉丧失（优势比 27.8[13.9 至 54.5]）；16.3%（6.8 至 30.7）的血清阳性个体无症状。在体外，对 43 名血清阳性者中的 31 名进行了血清中和作用阳性检测。鉴于血清流行率非常低，可以假设 2020 年 3 月的封锁对夏季有预防作用，但预计随后在该免疫不足的人群中会观察到第二波 COVID-19。
