Cesco Cassiele T, Valente Artur J M, Paulino Alexandre T
Department of Food and Chemical Engineering, Santa Catarina State University, Pinhalzinho 89870-000, Brazil.
Department of Chemistry, University of Coimbra, 3004-535 Coimbra, Portugal.
Pharmaceutics. 2021 Jun 7;13(6):842. doi: 10.3390/pharmaceutics13060842.
Chitosan/DNA blend hydrogel (CDB) and chitosan/pectin blend hydrogel (CPB) were synthesized using an emulsion (oil-in-water) technique for the release of methylene blue (model molecule). Both hydrogels were characterized by swelling assays, Fourier transform infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA) and scanning electron microscopy (SEM), before and after the methylene blue (MB) loading. Higher swelling degrees were determined for both hydrogels in simulated gastric fluid. FT-IR spectra inferred absorption peak changes and shifts after MB loading. The TGA results confirmed changes in the polymer network degradation. The SEM images indicated low porosities on the hydrogel surfaces, with deformed structure of the CPB. Smoother and more uniform surfaces were noticed on the CDB chain after MB loading. Higher MB adsorption capacities were determined at lower initial hydrogel masses and higher initial dye concentrations. The MB adsorption mechanisms on the hydrogel networks were described by the monolayer and multilayer formation. The MB release from hydrogels was studied in simulated gastric and intestinal fluids, at 25 °C and 37 °C, with each process taking place at roughly 6 h. Higher release rates were determined in simulated gastric fluid at 25 °C. The release kinetics of MB in chitosan/DNA and chitosan/pectin matrices follows a pseudo-second-order kinetic mechanism.
采用乳液（水包油）技术合成了壳聚糖/DNA共混水凝胶（CDB）和壳聚糖/果胶共混水凝胶（CPB），用于亚甲基蓝（模型分子）的释放。在负载亚甲基蓝（MB）前后，通过溶胀试验、傅里叶变换红外（FT-IR）光谱、热重分析（TGA）和扫描电子显微镜（SEM）对两种水凝胶进行了表征。在模拟胃液中测定了两种水凝胶的更高溶胀度。FT-IR光谱推断出负载MB后吸收峰的变化和位移。TGA结果证实了聚合物网络降解的变化。SEM图像表明水凝胶表面孔隙率低，CPB结构变形。负载MB后，在CDB链上观察到更光滑、更均匀的表面。在较低的初始水凝胶质量和较高的初始染料浓度下测定了更高的MB吸附容量。通过单层和多层形成描述了水凝胶网络上的MB吸附机制。在25℃和37℃下，在模拟胃液和肠液中研究了水凝胶中MB的释放，每个过程大约持续6小时。在25℃的模拟胃液中测定了更高的释放速率。壳聚糖/DNA和壳聚糖/果胶基质中MB的释放动力学遵循准二级动力学机制。
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