Department of Dental Materials and Prosthodontics, Institute of Science and Technology, Sao Paulo State University, UNESP, Sao Paulo 12.245-700, Brazil.
Department of Bioscience and Buccal Diagnose, Institute of Science and Technology, Sao Paulo State University, UNESP, Sao Paulo 12.245-700, Brazil.
Int J Mol Sci. 2020 Jun 26;21(12):4561. doi: 10.3390/ijms21124561.
Chitosan-DNA (CS-DNA) and Chitosan-Pectin (CS-P) hydrogels were formulated as a sustained drug delivery carrier for drug delivery. For this, hydrogels were prepared by emulsion technique: mixing aqueous phase of the CS and DNA or P solution with benzyl alcohol using a high-performance dispersing instrument. Green Propolis (GP) was incorporated by imbibition: hydrogels were placed in GP aqueous solution (70 µg/mL) for 2 h. The specimens were freeze-dried and then characterized using different techniques. In vitro cell viability and morphology were also performed using the MG63 cell line. The presence of P was evidenced by the occurrence of a strong band at 1745 cm, also occurring in the blend. DNA and CS-DNA showed a strong band at 1650 cm, slightly shifted from the chitosan band. The sorption of GP induced a significant modification of the gel surface morphology and some phase separation occurs between chitosan and DNA. Drug release kinetics in water and in saliva follow a two-step mechanism. Significant biocompatibility revealed that these hydrogels were non-toxic and provided acceptable support for cell survival. Thus, the hydrogel complexation of chitosan with DNA and with Pectin provides favorable micro-environment for cell growth and is a viable alternative drug delivery system for Green Propolis.
壳聚糖- DNA（CS-DNA）和壳聚糖-果胶（CS-P）水凝胶被设计为用于药物输送的药物输送的持续药物输送载体。为此，通过乳液技术制备水凝胶：使用高性能分散仪器将 CS 和 DNA 或 P 溶液的水相与苄醇混合。通过吸液将绿蜂胶（GP）掺入：将水凝胶置于 GP 水溶液（70μg/mL）中 2 小时。将样品冷冻干燥，然后使用不同的技术进行表征。还使用 MG63 细胞系进行体外细胞活力和形态学研究。存在 P 的证据是在 1745 cm 处出现强带，该带也存在于共混物中。CS-DNA 和 DNA 显示在 1650 cm 处的强带，与壳聚糖带略有位移。GP 的吸附诱导凝胶表面形态的显著修饰，并且壳聚糖和 DNA 之间发生一些相分离。在水中和唾液中的药物释放动力学遵循两步机制。显著的生物相容性表明这些水凝胶是无毒的，并为细胞存活提供了可接受的支持。因此，壳聚糖与 DNA 和果胶的水凝胶络合为细胞生长提供了有利的微环境，是绿蜂胶的可行的替代药物输送系统。
