Truong Son Dinh An, Tummanatsakun Doungdean, Proungvitaya Tanakorn, Limpaiboon Temduang, Wongwattanakul Molin, Chua-On Daraporn, Roytrakul Sittiruk, Proungvitaya Siriporn
Centre of Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
Cholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Diagnostics (Basel). 2021 Jun 8;11(6):1054. doi: 10.3390/diagnostics11061054.
The mortality rate of cholangiocarcinoma (CCA) is high since there is a lack of a non-invasive technique to accurately detect tumors at the early stage. CCA biomarkers are consistently needed for various purposes including screening, early diagnosis, prognosis and follow-up. Herein, using bioinformatic analysis of our mitochondrial proteome database of CCA tissues, we identified cytokine-induced apoptosis inhibitor 1 (CIAPIN1) as a potential prognostic biomarker for CCA. CIAPIN1 levels in the sera of 159 CCA patients and 93 healthy controls (HC) were measured using a dot blot assay. The median level ± quartile deviation of CIAPIN1 level in the sera of CCA patient group was 0.5144 ± 0.34 µg/µL, which was significantly higher than 0.2427 ± 0.09 µg/µL of the HC group ( < 0.0001). In CCA patients, higher serum CIAPIN1 level was significantly associated with lymph node metastasis ( = 0.024) and shorter overall survival time ( = 0.001, Kaplan-Meier test). Cox regression analysis showed that the serum CIAPIN1 level can be an independent prognostic indicator for the survival of CCA patients. Moreover, for the prediction of CCA prognosis, CIAPIN1 is superior to CEA, CA19-9 and ALP. In conclusion, CIAPIN1 can be a serum biomarker candidate for the poor prognosis of CCA.
胆管癌（CCA）的死亡率很高，因为缺乏一种能够在早期准确检测肿瘤的非侵入性技术。CCA生物标志物在包括筛查、早期诊断、预后评估和随访等各种目的中一直都是必需的。在此，通过对我们的CCA组织线粒体蛋白质组数据库进行生物信息学分析，我们鉴定出细胞因子诱导的凋亡抑制因子1（CIAPIN1）作为CCA潜在的预后生物标志物。使用斑点印迹法检测了159例CCA患者和93例健康对照（HC）血清中的CIAPIN1水平。CCA患者组血清中CIAPIN1水平的中位数±四分位数偏差为0.5144±0.34μg/μL，显著高于HC组的0.2427±0.09μg/μL（<0.0001）。在CCA患者中，血清CIAPIN1水平较高与淋巴结转移显著相关（=0.024），且总生存时间较短（=0.001，Kaplan-Meier检验）。Cox回归分析表明，血清CIAPIN1水平可以作为CCA患者生存的独立预后指标。此外，对于CCA预后的预测，CIAPIN1优于癌胚抗原（CEA）、糖类抗原19-9（CA19-9）和碱性磷酸酶（ALP）。总之，CIAPIN1可以作为CCA预后不良的血清生物标志物候选物。
