Matok Lital Ashtamkar, Azrad Maya, Leshem Tamar, Abuzahya Anan, Khamaisi Thanaa, Smolkin Tatiana, Peretz Avi
The Azrieli Faculty of Medicine, Bar-Ilan University, Safed 1311502, Israel.
Clinical Microbiology Laboratory, The Baruch Padeh Medical Center, Poriya, Tiberias 1311502, Israel.
Microorganisms. 2021 Jun 8;9(6):1245. doi: 10.3390/microorganisms9061245.
We evaluated carriage rates of extended spectrum β-lactam-producing (ESBL-E), Carbapeneme-resistant (CRE), vancomycin-resistant (VRE), and methicillin-resistant (MRSA) among pregnant women and determined the maternal-to-neonate transmission rates of these antibiotic-resistant bacteria (ARB). Pregnant women provided rectal and vaginal samples, proximal to delivery. Stool samples were collected from newborns within 48 h of birth. All samples were cultured on selective media for ARB identification. Clinical and demographic data were collected from the participants' medical files. We performed molecular and phenotypic characterization of the different resistance mechanisms, and determined the isolates' antibiotic susceptibility and biofilm-forming ability. The prevalence of ESBL-E, MRSA and VRE among pregnant women were 16%, 6% and 1%, respectively. The prevalence of ESBL-E and MRSA among neonates were 7.6% and 1.6%, respectively. Maternal-to-neonate transmission rates of ESBL-E and MRSA were 48% and 27.8%, respectively. Maternal and neonatal isolates shared similar characteristics. Maternal-to-neonate transmission of ARB plays an important role in bacterial colonization in newborns. Future studies should investigate the outcomes of the high ESBL-E transmission rate. The biofilm-forming ability of ARB was found to affect transmission. Additional factors should be investigated in order to understand the differences between transmitted and non-transmitted bacteria.
我们评估了孕妇中产超广谱β-内酰胺酶（ESBL-E）、耐碳青霉烯类（CRE）、耐万古霉素（VRE）和耐甲氧西林（MRSA）的携带率，并确定了这些耐药菌（ARB）的母婴传播率。孕妇在分娩前提供直肠和阴道样本。在新生儿出生后48小时内采集粪便样本。所有样本均在选择性培养基上培养以鉴定ARB。从参与者的医疗档案中收集临床和人口统计学数据。我们对不同耐药机制进行了分子和表型特征分析，并确定了分离株的抗生素敏感性和生物膜形成能力。孕妇中ESBL-E、MRSA和VRE的流行率分别为16%、6%和1%。新生儿中ESBL-E和MRSA的流行率分别为7.6%和1.6%。ESBL-E和MRSA的母婴传播率分别为48%和27.8%。母婴分离株具有相似的特征。ARB的母婴传播在新生儿细菌定植中起重要作用。未来的研究应调查ESBL-E高传播率的后果。发现ARB的生物膜形成能力会影响传播。为了解传播菌和非传播菌之间的差异，应研究其他因素。
