Hoffmann Tanja, Carsjens Imke, Rakotozandrindrainy Raphaël, Girmann Mirko, Randriamampionona Njary, Maïga-Ascofaré Oumou, Podbielski Andreas, Hahn Andreas, Frickmann Hagen, Schwarz Norbert Georg
Department of Microbiology and Hospital Hygiene, Bundeswehr Hospital Hamburg, 20359 Hamburg, Germany.
Department of Microbiology and Parasitology, University of Antananarivo, BP 566 Antananarivo, Madagascar.
Pathogens. 2021 Jun 8;10(6):722. doi: 10.3390/pathogens10060722.
This work was conducted as a cross sectional study to define the disease burden of schistosomiasis in pregnant Madagascan women and to evaluate serological and molecular diagnostic assays. A total of 1154 residual EDTA blood samples from pregnant Madagascan women were assessed. The nucleic acid extractions were subjected to in-house real-time PCRs specifically targeting complex, complex, and African spp. on genus level, while the EDTA plasma samples were analyzed using -specific IgG and IgM commercial ELISA and immunofluorescence assays. The analyses indicated an overall prevalence of schistosomiasis in Madagascan pregnant women of 40.4%, with only minor regional differences and differences between serology- and blood PCR-based surveillance. The specific real-time PCR showed superior sensitivity of 74% (specificity 80%) compared with the genus-specific real-time PCR (sensitivity 13%, specificity 100%) in blood. The laborious immunofluorescence (sensitivity IgM 49%, IgG 87%, specificity IgM 85%, IgG 96%) scored only slightly better than the automatable ELISA (sensitivity IgM 38%, IgG 88%, specificity IgM 78%, IgG 91%). Infections with were detected only. The high prevalence of schistosomiasis recorded here among pregnant women in Madagascar calls for actions in order to reduce the disease burden.
本研究作为一项横断面研究开展，旨在确定马达加斯加孕妇血吸虫病的疾病负担，并评估血清学和分子诊断检测方法。共评估了1154份马达加斯加孕妇的残留乙二胺四乙酸（EDTA）血样。核酸提取后进行了针对属水平上的复杂种、复杂种和非洲种的内部实时聚合酶链反应（PCR），而EDTA血浆样本则使用种特异性IgG和IgM商业酶联免疫吸附测定（ELISA）以及免疫荧光测定进行分析。分析表明，马达加斯加孕妇血吸虫病的总体患病率为40.4%，仅存在微小的地区差异以及基于血清学和血液PCR监测之间的差异。种特异性实时PCR在血液中的敏感性为74%（特异性为80%），优于属特异性实时PCR（敏感性13%，特异性100%）。费力的免疫荧光测定（IgM敏感性49%，IgG敏感性87%，IgM特异性85%，IgG特异性96%）的得分仅略高于可自动化的ELISA（IgM敏感性38%，IgG敏感性88%，IgM特异性78%，IgG特异性91%）。仅检测到感染。马达加斯加孕妇中血吸虫病的高患病率要求采取行动以减轻疾病负担。
