Okba Mona M, El-Shiekh Riham A, Abu-Elghait Mohammed, Sobeh Mansour, Ashour Rehab M S
Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt.
Department of Botany and Microbiology, Faculty of Science, Al-azhar University, Cairo 11884, Egypt.
Antibiotics (Basel). 2021 Jun 23;10(7):761. doi: 10.3390/antibiotics10070761.
The development of multidrug-resistant bacterial strains is a worldwide emerging problem that needs a global solution. Exploring new natural antibiofilm agents is one of the most important alternative therapies in combating bacterial infections. This study aimed at testing the antimicrobial potential of flowers extract (ESFE) against , , , and prior to testing the antibiofilm activity against , and . ESFE demonstrated antimicrobial activity and promising inhibition activity against methicillin-resistant (MRSA) biofilm formation up to 95.9% ( < 0.05) at a concentration of 0.05 mg/mL and eradicated biofilm formation up to 71.2% ( < 0.05) at a concentration of 0.7 mg/mL. LC-MS analysis allowed the tentative identification of eighty-three secondary metabolites: 21 phloroglucinol, 18 terpenes, 16 flavonoids, 7 oleuropeic acid derivatives, 7 ellagic acid derivatives, 6 gallic acid derivatives, 3 phenolic acids, 3 fatty acids and 2 miscellaneous. In conclusion, is a rich source of effective constituents that promote its valorization as a promising candidate in the management of multidrug-resistant bacterial infections.
多重耐药细菌菌株的出现是一个全球性的新问题，需要全球共同解决。探索新型天然抗生物膜剂是对抗细菌感染最重要的替代疗法之一。本研究旨在测试花朵提取物（ESFE）对金黄色葡萄球菌、表皮葡萄球菌、粪肠球菌、屎肠球菌和耐甲氧西林金黄色葡萄球菌（MRSA）的抗菌潜力，然后再测试其对铜绿假单胞菌、鲍曼不动杆菌和白色念珠菌的抗生物膜活性。ESFE表现出抗菌活性，在浓度为0.05mg/mL时，对耐甲氧西林金黄色葡萄球菌（MRSA）生物膜形成的抑制活性高达95.9%（P<0.05），在浓度为0.7mg/mL时，对金黄色葡萄球菌生物膜形成的消除率高达71.2%（P<0.05）。液相色谱-质谱联用（LC-MS）分析初步鉴定出83种次生代谢产物：21种间苯三酚、18种萜类、16种黄酮类、7种橄榄酸衍生物、7种鞣花酸衍生物、6种没食子酸衍生物、3种酚酸、3种脂肪酸和2种其他类。总之，[具体植物名称未给出]是有效成分的丰富来源，这使其作为治疗多重耐药细菌感染的有前景候选物具有很高的价值。
