Xie Xing-Huan, Fu Xin, Yan Xing-Yu, Peng Wen-Fang, Kang Li-Xin
State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei University, Wuhan 430072, China.
Mar Drugs. 2021 Jun 23;19(7):356. doi: 10.3390/md19070356.
k10 isolated from soil revealed the hydrolyzing ability of shrimp chitin and antifungal activity against The k10 chitinase was produced from a powder chitin-containing medium and purified by ammonium sulfate precipitation and column chromatography. The purified chitinase showed maximal activity toward colloidal chitin at pH 5 and 40 °C. The enzymatic activity was enhanced by potassium and zinc, and it was inhibited by silver, iron, and copper. The chitinase could convert colloidal chitin to N-acetylglucosamine (GlcNAc), (GlcNAc)2, and (GlcNAc)3, showing that this enzyme had endocleavage and exocleavage activities. In addition, the chitinase prevented the mycelial growth of the phytopathogenic fungi and These results indicate that k10 is a potential candidate for producing chitinase that could be useful for generating chitooligosaccharides from chitinous waste and functions as a fungicide.
从土壤中分离出的K10显示出对虾几丁质的水解能力以及对[此处原文缺失两种真菌名称]的抗真菌活性。K10几丁质酶由含粉末状几丁质的培养基产生，并通过硫酸铵沉淀和柱色谱法进行纯化。纯化后的几丁质酶在pH值为5和40℃时对胶体几丁质表现出最大活性。钾和锌可增强酶活性，而银、铁和铜则对其有抑制作用。该几丁质酶可将胶体几丁质转化为N-乙酰葡糖胺（GlcNAc）、（GlcNAc）2和（GlcNAc）3，表明该酶具有内切和外切活性。此外，该几丁质酶可抑制植物病原真菌[此处原文缺失两种真菌名称]的菌丝生长。这些结果表明，K10是生产几丁质酶的潜在候选物，该几丁质酶可用于从含几丁质的废料中生成壳寡糖，并具有杀菌剂的功能。
