Dascalu Rusu Laura Monica, Moldovan Marioara, Prodan Doina, Ciotlaus Irina, Popescu Violeta, Baldea Ioana, Carpa Rahela, Sava Sorina, Chifor Radu, Badea Mindra Eugenia
Department of Prosthodontics and Dental Materials, Iuliu Hatieganu University of Medicine and Pharmacy, 31 Avram Iancu Str, 400083 Cluj-Napoca, Romania.
Institute of Chemistry Raluca Ripan, Babes-Bolyai University, 30 Fantanele Str, 400294 Cluj-Napoca, Romania.
Materials (Basel). 2020 Jul 6;13(13):3012. doi: 10.3390/ma13133012.
The novelty of this study consists on the formulation and evaluation of five complex experimental natural photosensitizers (PS): gel with oregano essential oil (O), gel with methylene blue (AM), gel with a mixture of essential oils (Thieves-H), gel with arnica oil and curcuma extract (CU) and gel with frankincense essential oil (T), used as photosensitizing agents (PS) in antimicrobial photodynamic therapy (aPDT) in the control of microbial biofilm in oral cavity. The experimental PS were characterized by gas chromatography-mass spectrometry (GC-MS), Fourier-transform infrared spectroscopy (FTIR), UV-Vis spectroscopy, cytotoxicity assay, antimicrobial effect and scanning electron microscopy (SEM). The IR spectra of the experimental PS with essential oils exhibit absorption bands due to the presence of water and glycerol in high quantities. The studied compounds had a reduced cytotoxic effect on cell cultures. The lowest cytotoxic effect was observed in experimental PS with oregano essential oil and methylene blue PS. Essential oils with proven antibacterial capabilities used in experimental PS confer antibacterial activity to the gels in which they are incorporated, an activity that may be more efficient use of a PDT therapy. Single bacteria were detected mainly by SEM after 12 h, while aggregate bacteria and micro colonies dominated the samples at 48 h.
本研究的新颖之处在于配制并评估了五种复合实验性天然光敏剂（PS）：含牛至精油的凝胶（O）、含亚甲蓝的凝胶（AM）、含混合精油的凝胶（Thieves-H）、含山金车油和姜黄提取物的凝胶（CU）以及含乳香精油的凝胶（T），这些光敏剂在口腔微生物生物膜控制的抗菌光动力疗法（aPDT）中用作光敏剂（PS）。通过气相色谱 - 质谱联用（GC-MS）、傅里叶变换红外光谱（FTIR）、紫外 - 可见光谱、细胞毒性测定、抗菌效果和扫描电子显微镜（SEM）对实验性PS进行了表征。含精油的实验性PS的红外光谱由于大量水和甘油的存在而呈现吸收带。所研究的化合物对细胞培养物的细胞毒性作用降低。在含牛至精油和亚甲蓝PS的实验性PS中观察到最低的细胞毒性作用。实验性PS中使用的具有已证实抗菌能力的精油赋予了它们所掺入凝胶抗菌活性，这种活性可能使光动力疗法的使用更有效。12小时后通过SEM主要检测到单个细菌，而在48小时时聚集细菌和微菌落占主导。
