Rudolph R, Vande Berg J, Schneider J A, Fisher J C, Poolman W L
San Diego Veterans Administration Medical Center, La Jolla, Calif.
Plast Reconstr Surg. 1988 Oct;82(4):669-77. doi: 10.1097/00006534-198810000-00019.
To study radiation effect separate from the microcirculation, fibroblasts were cultured from four patients with radiation wounds. Cells could be grown from irradiated tissue near the ulcer and from control normal tissue, but no cells could be cultured from the ulcers. The ability of radiation-treated fibroblasts to attach to the substrate and form colonies was less than that of unirradiated cells. Irradiated skin fibroblasts from the four patients had significantly longer mean generation times than did control cells. During log-phase growth (1 to 9 days), the population doublings of damaged cells were significantly reduced compared to colonies from normal cells. These data suggest a permanent intrinsic radiation effect on fibroblasts or a selective ablation of faster-growing fibroblast subpopulations that is not dependent on decreased blood supply.
为了研究独立于微循环的辐射效应，从四名患有放射性伤口的患者身上培养成纤维细胞。细胞可以从溃疡附近的受辐射组织以及对照正常组织中生长出来，但无法从溃疡处培养出细胞。经辐射处理的成纤维细胞附着于底物并形成集落的能力低于未受辐射的细胞。来自这四名患者的受辐射皮肤成纤维细胞的平均代时明显长于对照细胞。在对数生长期（1至9天），与正常细胞的集落相比，受损细胞的群体倍增明显减少。这些数据表明，辐射对成纤维细胞具有永久性的内在效应，或者对生长较快的成纤维细胞亚群进行了选择性消融，这并不依赖于血液供应的减少。
