Division of Plastic and Reconstructive Surgery, Keck School of Medicine of USC, 1510 San Pablo St., Suite 415, Los Angeles, CA, 90033, USA.
Department of Surgery, Keck School of Medicine of USC, 1510 San Pablo St., Suite 415, Los Angeles, CA, 90033, USA.
Sci Rep. 2017 Jun 20;7(1):3876. doi: 10.1038/s41598-017-03614-y.
Wound healing is significantly delayed in irradiated skin. To better understand global changes in protein expression after radiation, we utilized a reverse phase protein array (RPPA) to identify significant changes in paired samples of normal and irradiated human skin. Of the 210 proteins studied, fibronectin was the most significantly and consistently downregulated in radiation-damaged skin. Using a murine model, we confirmed that radiation leads to decreased fibronectin expression in the skin as well as delayed wound healing. Topically applied fibronectin was found to significantly improve wound healing in irradiated skin and was associated with decreased inflammatory infiltrate and increased angiogenesis. Fibronectin treatment may be a useful adjunctive modality in the treatment of non-healing radiation wounds.
辐射会显著延迟皮肤伤口愈合。为了更好地理解辐射后蛋白质表达的全局变化，我们利用反相蛋白阵列（RPPA）鉴定正常和辐射损伤的人皮肤配对样本中的显著变化。在研究的 210 种蛋白质中，纤维连接蛋白在辐射损伤的皮肤中下调最显著且一致。通过使用小鼠模型，我们证实辐射会导致皮肤中纤维连接蛋白表达减少，以及伤口愈合延迟。局部应用纤维连接蛋白可显著改善辐射损伤皮肤的伤口愈合，与减少炎症浸润和增加血管生成有关。纤维连接蛋白治疗可能是治疗非愈合性放射性伤口的一种有用的辅助治疗方法。
