Department of Diagnostic and Interventional Radiology, University Hospital of Tübingen, D-72076 Tübingen, Germany.
Department of Orthopedic Surgery, University Hospital of Tübingen, D-72076 Tübingen, Germany.
Int J Mol Sci. 2021 Jun 25;22(13):6831. doi: 10.3390/ijms22136831.
Mesenchymal stem cells (MSC) are known for their vascular regeneration capacity by neoangiogenesis. Even though, several delivery approaches exist, particularly in the case of intravascular delivery, only limited number of cells reach the targeted tissue and are not able to remain on site. Applicated cells exhibit poor survival accompanied with a loss of functionality. Moreover, cell application techniques lead to cell death and impede the overall MSC function and survival. 3D cell spheroids mimic the physiological microenvironment, thus, overcoming these limitations. Therefore, in this study we aimed to evaluate and assess the feasibility of 3D MSCs spheroids for endovascular application, for treatment of ischemic peripheral vascular pathologies. Multicellular 3D MSC spheroids were generated at different cell seeding densities, labelled with ultra-small particles of iron oxide (USPIO) and investigated in vitro in terms of morphology, size distribution, mechanical stability as well as ex vivo with magnetic resonance imaging (MRI) to assess their trackability and distribution. Generated 3D spheroids were stable, viable, maintained stem cell phenotype and were easily trackable and visualized via MRI. MSC 3D spheroids are suitable candidates for endovascular delivery approaches in the context of ischemic peripheral vascular pathologies.
间充质干细胞 (MSC) 以其新生血管形成的血管再生能力而闻名。尽管存在多种输送方法，特别是在血管内输送的情况下，只有有限数量的细胞能够到达靶向组织，并且无法留在原位。应用的细胞表现出较差的存活率，同时伴随着功能丧失。此外，细胞应用技术会导致细胞死亡，从而阻碍 MSC 的整体功能和存活。3D 细胞球体模拟生理微环境，从而克服这些限制。因此，在这项研究中，我们旨在评估和评估 3D MSC 球体用于血管内应用的可行性，以治疗缺血性周围血管病变。多细胞 3D MSC 球体在不同的细胞接种密度下生成，用超小氧化铁颗粒 (USPIO) 标记，并在体外从形态、大小分布、机械稳定性等方面进行研究，以及通过磁共振成像 (MRI) 进行离体研究，以评估其可追踪性和分布情况。生成的 3D 球体稳定、有活力、保持干细胞表型，并且可以通过 MRI 轻松追踪和可视化。MSC 3D 球体是缺血性周围血管病变血管内输送方法的合适候选者。
