Wißmann Rebecca, Martirosian Petros, Danalache Marina, Grözinger Gerd, Schick Fritz, Elser Stefanie
Department of Diagnostic and Interventional Radiology, University Hospital of Tübingen, D-72076, Tübingen, Germany.
Section on Experimental Radiology, Department of Diagnostic and Interventional Radiology, Tübingen University Hospital, Tübingen, Germany.
Heliyon. 2025 Jan 17;11(2):e41803. doi: 10.1016/j.heliyon.2025.e41803. eCollection 2025 Jan 30.
Over the past decade, significant progress has been made in the utilization of three-dimensional cell cultures in the form of spheroids as a bridge between and vivo models. This is contributed by natural cell-cell interactions that occur within spheroids, leading to the subsequent development of extracellular matrix. The assessment of cell spheroids with conventional microscopy is destructive, requiring sectioning that damages their micro-structures. To address these issues, we developed and propose a non-invasive approach using magnetic resonance imaging (MRI). Despite its limited spatial resolution, this method adeptly reveals information about the composition and vitality of stem cell and cancer spheroids and their micro-environment in a non-invasive manner. This work reports on the development of an MRI-compatible setup for culturing cell spheroids, tailored for use with standard 3 T whole-body MRI systems. Systematic studies with different cell types show the potential of the proposed approach for simultaneous actuation and visualization of cell spheroids, with potential across a broad spectrum of applications.
在过去十年中，以球体形式存在的三维细胞培养物作为体外和体内模型之间的桥梁，在其利用方面取得了重大进展。这得益于球体内部发生的自然细胞间相互作用，进而导致细胞外基质的后续形成。用传统显微镜评估细胞球体具有破坏性，需要切片，这会损害其微观结构。为了解决这些问题，我们开发并提出了一种使用磁共振成像（MRI）的非侵入性方法。尽管其空间分辨率有限，但该方法能够以非侵入性方式巧妙地揭示有关干细胞和癌症球体及其微环境的组成和活力的信息。这项工作报告了一种用于培养细胞球体的MRI兼容装置的开发，该装置专为与标准3T全身MRI系统配合使用而设计。对不同细胞类型的系统研究表明，所提出的方法具有同时驱动和可视化细胞球体的潜力，在广泛的应用中具有潜力。
