Tlak Gajger Ivana, Šimenc Laura, Toplak Ivan
Department for Biology and Pathology of Fish and Bees, Faculty of Veterinary Medicine, University of Zagreb, 10000 Zagreb, Croatia.
Virology Unit, Institute of Microbiology and Parasitology, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, Slovenia.
Pathogens. 2021 Jun 25;10(7):808. doi: 10.3390/pathogens10070808.
To determine the presence and the prevalence of four different honeybee viruses (acute bee paralysis virus-ABPV, black queen cell virus-BQCV, chronic bee paralysis virus-CBPV, deformed wing virus-DWV) in wild bumblebees, pooled randomly selected bumblebee samples were collected from twenty-seven different locations in the territory of Croatia. All samples were prepared and examined using the RT-PCR methods for quantification of mentioned honeybee viruses. Determined prevalence (%) of identified positive viruses were in the following decreasing order: BQCV > DWV > ABPV, CBPV. Additionally, direct sequencing of samples positive for BQCV ( = 24) and DWV ( = 2) was performed, as well as a test of molecular phylogeny comparison with those available in GenBank. Selected positive field viruses' strains showed 95.7 to 100% (BQCV) and 98.09% (DWV) nucleotide identity with previously detected and deposited honeybee virus strains in the geographic areas in Croatia and neighboring Slovenia. In this article, the first detection of four honeybee viruses with genetic characterization of high diversity strains circulating in wild bumblebees in Croatia is presented.
为了确定四种不同蜜蜂病毒（急性蜜蜂麻痹病毒 - ABPV、黑蜂王台病毒 - BQCV、慢性蜜蜂麻痹病毒 - CBPV、残翅病毒 - DWV）在野生熊蜂中的存在情况和流行率，从克罗地亚境内27个不同地点随机采集了熊蜂样本并进行汇总。所有样本均采用逆转录聚合酶链反应（RT-PCR）方法进行制备和检测，以定量上述蜜蜂病毒。已鉴定出的阳性病毒的确定流行率（%）按以下降序排列：BQCV > DWV > ABPV、CBPV。此外，对BQCV（n = 24）和DWV（n = 2）阳性的样本进行了直接测序，并与GenBank中现有的序列进行了分子系统发育比较测试。所选的阳性野外病毒株与克罗地亚及邻国斯洛文尼亚地理区域中先前检测和保存的蜜蜂病毒株的核苷酸同一性为95.7%至100%（BQCV）和98.09%（DWV）。本文首次报道了在克罗地亚野生熊蜂中检测到四种蜜蜂病毒，并对高多样性毒株进行了遗传特征分析。
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