鉴定熊蜂阳性样本中四种蜜蜂病毒的遗传同源株。
Determination of Genetically Identical Strains of Four Honeybee Viruses in Bumblebee Positive Samples.
机构信息
Institute of Microbiology and Parasitology, Virology Unit, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, Slovenia.
Institute of Pathology, Wild Animals, Fish and Bees, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, 1000 Ljubljana, Slovenia.
出版信息
Viruses. 2020 Nov 16;12(11):1310. doi: 10.3390/v12111310.
In recent years, there has been growing evidence that certain types of honeybee viruses could be transmitted between different pollinators. Within a voluntary monitoring programme, 180 honeybee samples () were collected from affected apiaries between 2007 and 2018. Also from August 2017 to August 2018, a total 148 samples of healthy bumblebees () were collected at four different locations in Slovenia, and all samples were tested by using RT-PCR methods for six honeybee viruses. Direct sequencing of a total 158 positive samples (acute bee paralysis virus (ABPV = 33), black queen cell virus (BQCV = 75), sacbrood bee virus (SBV = 25) and Lake Sinai virus (LSV = 25)) was performed from obtained RT-PCR products. The genetic comparison of identified positive samples of bumblebees and detected honeybee field strains of ABPV, BQCV, SBV, and LSV demonstrated 98.74% to 100% nucleotide identity between both species. This study not only provides evidence that honeybees and bumblebees are infected with genetically identical or closely related viral strains of four endemically present honeybee viruses but also detected a high diversity of circulating strains in bumblebees, similar as was observed among honeybees. Important new genetic data for endemic strains circulating in honeybees and bumblebees in Slovenia are presented.
近年来，越来越多的证据表明，某些类型的蜜蜂病毒可能在不同的传粉媒介之间传播。在一项自愿监测计划中，2007 年至 2018 年间从受影响的蜂场采集了 180 个蜜蜂样本。2017 年 8 月至 2018 年 8 月期间，还在斯洛文尼亚的四个不同地点采集了总共 148 只健康熊蜂样本，并使用 RT-PCR 方法对这 148 只熊蜂样本进行了六种蜜蜂病毒的检测。对总共 158 个阳性样本（急性麻痹病毒 (ABPV = 33)、黑蜂王细胞病毒 (BQCV = 75)、囊状幼虫病病毒 (SBV = 25) 和 Sinai 湖病毒 (LSV = 25)）的 RT-PCR 产物进行了直接测序。从获得的 RT-PCR 产物中直接测序。对鉴定的熊蜂阳性样本和检测到的 ABPV、BQCV、SBV 和 LSV 等四种地方性蜜蜂田间毒株的遗传比较表明，两种物种之间的核苷酸同一性为 98.74%至 100%。本研究不仅提供了证据表明蜜蜂和熊蜂感染了具有相同遗传或密切相关的四种地方性蜜蜂病毒的病毒株，而且还检测到了熊蜂中循环株的高度多样性，这与在蜜蜂中观察到的情况相似。本文为斯洛文尼亚蜜蜂和熊蜂中循环的地方性株提供了重要的新遗传数据。
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