Socha Piotr, Bladowska Katarzyna, Zduńczyk Sławomir, Janowski Tomasz
Department of Animal Reproduction with Clinic, University of Warmia and Mazury, Oczapowskiego 14, 10-719 Olsztyn, Poland.
Animals (Basel). 2021 Jun 28;11(7):1922. doi: 10.3390/ani11071922.
The aim of the study was to evaluate the influence of administration of aglepristone in mid-proestrus on progesterone concentration, LH release, and occurrence of ovulation in the bitch. Experimental bitches ( = 7) were treated on days 4 and 5 of proestrus with aglepristone at the dose of 10 mg/kg body weight s.c. (i.e., the two treatments were 24 h apart). Control animals ( = 7) received s.c. injections of saline. For progesterone determination, blood was collected daily until the first day of cytological diestrus. For LH determination, blood was collected daily and in the periovulatory phase every 8 h. The progesterone concentration showed a similar pattern in both groups. The LH peak value in bitches treated with aglepristone was significantly lower ( < 0.05) than that in control bitches (4.83 ± 1.20 vs. 13.66 ± 1.21 ng/mL). The area under the curve (AUC) for LH was significantly ( < 0.05) lower in treated than in control animals (6.85 ± 1.21 ng/mL/d vs. 12.25 ± 1.35 ng/mL/d). The ovulation occurred in all animals in both groups. The study showed that administration of aglepristone in the mid-proestrus significantly reduced the preovulatory LH surge, but it had no effect on progesterone concentration and the occurrence of ovulation.
本研究的目的是评估在发情前期中期给予阿格列司酮对母犬孕酮浓度、促黄体生成素（LH）释放及排卵发生情况的影响。实验母犬（n = 7）在发情前期的第4天和第5天接受皮下注射阿格列司酮，剂量为10 mg/kg体重（即两次治疗间隔24小时）。对照动物（n = 7）接受皮下注射生理盐水。为测定孕酮，每天采集血液直至细胞学黄体期的第一天。为测定LH，每天采集血液，并在排卵前期每8小时采集一次。两组的孕酮浓度呈现相似模式。用阿格列司酮治疗的母犬的LH峰值显著低于（P < 0.05）对照母犬（4.83 ± 1.20 vs. 13.66 ± 1.21 ng/mL）。治疗组LH的曲线下面积（AUC）显著低于（P < 0.05）对照动物（6.85 ± 1.21 ng/mL/d vs. 12.25 ± 1.35 ng/mL/d）。两组所有动物均发生排卵。该研究表明，在发情前期中期给予阿格列司酮可显著降低排卵前LH峰，但对孕酮浓度和排卵发生无影响。
