Dipartimento di Patologia, Clinica e Diagnostica Veterinaria, Sezione di Ostetricia e Ginecologia, Università di Perugia, via S. Costanzo 4, Perugia I-06123, Italy.
Theriogenology. 2010 Sep 1;74(4):672-81. doi: 10.1016/j.theriogenology.2010.03.021. Epub 2010 May 7.
The effect of the antiprogestagen aglepristone (10 mg/kg bw), administered at days 29 and 30 following the estimated day of LH surge (day 0), on corpora lutea (CL) function was examined during the diestrus phase of non-pregnant bitches. Aglepristone shortened (P < 0.01) the luteal phase and complete luteolysis (progesterone <2 ng/mL) was observed at days 40.8 +/- 3.5 and 71.5 +/- 4.6 (means +/- SD; n = 9/group) in treated and control bitches, respectively. Peripheral estradiol-17beta concentrations declined from 91.5 +/- 14.3 pg/mL at day 9 to 50 pg/mL at day 18, remaining at approximately the same levels thereafter in both treated and control bitches. Intraluteal in vitro synthesis of progesterone and estradiol-17beta released by CL explanted at day 38 from control bitches (511.9 +/- 285.6 and 40.7 +/- 17.2 pg/mg protein, respectively) did not differ from that of treated. From day 38, intraovarian hemodynamic variables (arterial blood flow, systolic peak, and end-diastolic velocities), monitored by color-coded and pulsed Doppler, decreased more steeply (P < 0.01) in aglepristone-treated (n = 4) than in control (n = 4) bitches, whereas the resistance index increased (P < 0.01) in treated animals. All the blood flow parameters were undetectable at 60 +/- 3.6 and 68 +/- 2.0 days (medians +/- SD) after LH peak in treated and control bitches, respectively. In conclusion, aglepristone administration to dogs during the mid-luteal phase markedly accelerates the luteolytic process which is accompanied by a parallel decline in ovarian blood flow supply with a shift from approximately 8 to 10 days.
在非妊娠母犬的发情周期后期，用抗孕激素 aglepristone（10mg/kg bw）在估计 LH 峰日后 29 和 30 天（第 0 天）给药，研究其对黄体（CL）功能的影响。Aglepristone 缩短了黄体期（P<0.01），并且在处理和对照犬中分别在第 40.8±3.5 和 71.5±4.6 天观察到完全黄体溶解（孕酮<2ng/mL）。外周雌二醇-17β浓度从第 9 天的 91.5±14.3pg/mL 下降到第 18 天的 50pg/mL，此后在处理和对照犬中均保持在大致相同的水平。从第 38 天开始，从对照犬中提取的黄体（CL）的体外孕激素和雌二醇-17β的合成（分别为 511.9±285.6 和 40.7±17.2pg/mg 蛋白）在处理组和对照组之间没有差异。从第 38 天开始，通过彩色编码和脉冲多普勒监测的卵巢内血流动力学变量（动脉血流、收缩峰和舒张末期速度）在 aglepristone 处理（n=4）犬中下降得更陡峭（P<0.01），而在处理动物中阻力指数增加（P<0.01）。在 LH 峰后 60±3.6 和 68±2.0 天（中位数±SD），在处理和对照犬中，所有血流参数均无法检测到。总之，在黄体中期给犬用 aglepristone 给药可显著加速黄体溶解过程，同时卵巢血流供应平行下降，大约从 8 天左右转变为 10 天左右。
