Gofman Iosif V, Nikolaeva Alexandra L, Khripunov Albert K, Ivan'kova Elena M, Shabunin Anton S, Yakimansky Alexander V, Romanov Dmitriy P, Popov Anton L, Ermakov Artem M, Solomevich Sergey O, Bychkovsky Pavel M, Baranchikov Alexander E, Ivanov Vladimir K
Institute of Macromolecular Compounds, Russian Academy of Sciences, 199004 Saint Petersburg, Russia.
H. Turner National Medical Research Center for Children's Orthopedics and Trauma Surgery, Pushkin, 196603 Saint Petersburg, Russia.
Polymers (Basel). 2021 Jun 18;13(12):1999. doi: 10.3390/polym13121999.
A technique for the fabrication of bacterial cellulose-based films with CeO nanofiller has been developed. The structural and morphological characteristics of the materials have been studied, their thermal and mechanical properties in dry and swollen states having been determined. The preparation methodology makes it possible to obtain composites with a uniform distribution of nanoparticles. The catalytic effect of ceria, regarding the thermal oxidative destruction of cellulose, has been confirmed by TGA and DTA methods. An increase in CeO content led to an increase in the elastic modulus (a 1.27-fold increase caused by the introduction of 5 wt.% of the nanofiller into the polymer) and strength of the films. This effect is explained by the formation of additional links between polymer macro-chains via the nanoparticles' surface. The materials fabricated were characterized by a limited ability to swell in water. Swelling caused a 20- to 30-fold reduction in the stiffness of the material, the mechanical properties of the films in a swollen state remaining germane to their practical use. The application of the composite films in cell engineering as substrates for the stem cells' proliferation has been studied. The increase in CeO content in the films enhanced the proliferative activity of embryonic mouse stem cells. The cells cultured on the scaffold containing 5 wt.% of ceria demonstrated increased cell survival and migration activity. An analysis of gene expression confirmed improved cultivation conditions on CeO-containing scaffolds.
一种制备含CeO纳米填料的细菌纤维素基薄膜的技术已被开发出来。已对材料的结构和形态特征进行了研究，测定了它们在干燥和溶胀状态下的热性能和力学性能。该制备方法能够获得纳米颗粒均匀分布的复合材料。通过热重分析（TGA）和差示热分析（DTA）方法证实了二氧化铈对纤维素热氧化破坏的催化作用。CeO含量的增加导致薄膜的弹性模量增加（将5 wt.%的纳米填料引入聚合物中导致弹性模量增加1.27倍）以及强度增加。这种效应是通过纳米颗粒表面在聚合物大分子链之间形成额外的连接来解释的。所制备的材料在水中的溶胀能力有限。溶胀使材料的刚度降低了20至30倍，溶胀状态下薄膜的力学性能与其实际应用相关。已研究了复合薄膜在细胞工程中作为干细胞增殖底物的应用。薄膜中CeO含量的增加增强了小鼠胚胎干细胞的增殖活性。在含有5 wt.%二氧化铈的支架上培养的细胞表现出细胞存活率和迁移活性的增加。基因表达分析证实了含CeO支架上培养条件的改善。
