Department of Pediatrics, Gastroenterology, Hepatology, Nutrition and Allergology, Medical University of Bialystok, ul. Waszyngtona 17, 15-274 Bialystok, Poland.
Department of Pediatric Emergency Medicine, Medical University of Bialystok, ul. Waszyngtona 17, 15-274 Bialystok, Poland.
Biomolecules. 2021 Jun 18;11(6):911. doi: 10.3390/biom11060911.
Underage drinking is associated with health risk behaviors. Serum keratin-18 (CK18) levels are increased in liver diseases and may be biomarkers of outcome. The purpose of this study was to determine if the total CK18 (M65) or caspase-cleaved CK18 (M30) levels were different in adolescents admitted to hospital because of alcohol intoxication and controls with excluded liver diseases.
A prospective study included 57 adolescents after alcohol use and 23 control subjects. The concentrations of M30 and M65 in the serum samples were evaluated using an enzyme-linked immunosorbent assay.
The median age was 15 (14-17) years and 49% were male. There were significant differences in M65 levels between the study and control groups ( = 0.03). The concentrations of M30 and M65 were insignificant in adolescents divided into subgroups according to blood alcohol concentrations (BAC). Significant positive correlations were found between BAC and M65 levels ( = 0.038; r = 0.3). In receiver operating characteristic (ROC) analysis M65 (cut-off = 125.966 IU/l, Se = 70.2%, Sp = 43.5%) allowed to differentiate between patients with and without alcohol intoxication (AUC = 0.66, = 0.03).
M65 appears to be a promising non-invasive biomarker of hepatocyte injury during alcohol intoxication in adolescents. Moreover, a higher concentration of M65 may indicate early organ injury before the increase in the activity of liver enzymes, alanine aminotransferase (ALT) and aspartate aminotransferase (AST).
未成年人饮酒与健康风险行为有关。血清角蛋白 18（CK18）水平在肝脏疾病中升高，可能是预后的生物标志物。本研究旨在确定因酒精中毒住院的青少年和排除肝脏疾病的对照组之间，总 CK18（M65）或半胱氨酸天冬氨酸蛋白酶切割 CK18（M30）的水平是否不同。
一项前瞻性研究纳入了 57 名因酒精使用而住院的青少年和 23 名对照者。使用酶联免疫吸附试验评估血清样本中 M30 和 M65 的浓度。
中位年龄为 15 岁（14-17 岁），49%为男性。研究组和对照组之间 M65 水平存在显著差异（ = 0.03）。根据血液酒精浓度（BAC）将青少年分为亚组后，M30 和 M65 的浓度无显著性差异。BAC 与 M65 水平之间存在显著正相关（ = 0.038；r = 0.3）。在受试者工作特征（ROC）分析中，M65（截断值 = 125.966 IU/l，Se = 70.2%，Sp = 43.5%）可区分酒精中毒患者和非酒精中毒患者（AUC = 0.66， = 0.03）。
M65 似乎是青少年酒精中毒期间肝细胞损伤的一种有前途的非侵入性生物标志物。此外，M65 浓度较高可能表明在丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）等肝酶活性增加之前，就已经出现了早期器官损伤。
