Delivopoulos Evangelos, Murray Alan F, MacLeod Nikki K, Curtis John C
Centre for Integrative Physiology, School of Biomedical Sciences, The University of Edinburgh, Hugh Robson Building, George Square, Edinburgh, United Kingdom.
Biomaterials. 2009 Apr;30(11):2048-58. doi: 10.1016/j.biomaterials.2008.12.049. Epub 2009 Jan 12.
This paper describes a simple technique for the patterning of glia and neurons. The integration of neuronal patterning to Multi-Electrode Arrays (MEAs), planar patch clamp and silicon based 'lab on a chip' technologies necessitates the development of a microfabrication-compatible method, which will be reliable and easy to implement. In this study a highly consistent, straightforward and cost effective cell patterning scheme has been developed. It is based on two common ingredients: the polymer parylene-C and horse serum. Parylene-C is deposited and photo-lithographically patterned on silicon oxide (SiO(2)) surfaces. Subsequently, the patterns are activated via immersion in horse serum. Compared to non-activated controls, cells on the treated samples exhibited a significantly higher conformity to underlying parylene stripes. The immersion time of the patterns was reduced from 24 to 3h without compromising the technique. X-ray photoelectron spectroscopy (XPS) analysis of parylene and SiO(2) surfaces before and after immersion in horse serum and gel based eluant analysis suggests that the quantity and conformation of proteins on the parylene and SiO(2) substrates might be responsible for inducing glial and neuronal patterning.
本文描述了一种用于胶质细胞和神经元图案化的简单技术。将神经元图案化整合到多电极阵列（MEA）、平面膜片钳和基于硅的“芯片实验室”技术中，需要开发一种与微制造兼容的方法，该方法要可靠且易于实施。在本研究中，已开发出一种高度一致、直接且具有成本效益的细胞图案化方案。它基于两种常见成分：聚对二甲苯-C聚合物和马血清。聚对二甲苯-C沉积在氧化硅（SiO₂）表面并通过光刻进行图案化。随后，通过浸入马血清来激活图案。与未激活的对照相比，处理过的样品上的细胞对下面的聚对二甲苯条纹表现出明显更高的一致性。图案的浸入时间从24小时减少到3小时，而不影响该技术。对浸入马血清前后的聚对二甲苯和SiO₂表面进行X射线光电子能谱（XPS）分析以及基于凝胶的洗脱液分析表明，聚对二甲苯和SiO₂底物上蛋白质的数量和构象可能是诱导胶质细胞和神经元图案化的原因。
