Morcillo Rafael J L, Vílchez Juan I, Zhang Song, Kaushal Richa, He Danxia, Zi Hailing, Liu Renyi, Niehaus Karsten, Handa Avtar K, Zhang Huiming
Shanghai Center for Plant Stress Biology, Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, China.
Institute for Water Research and Department of Microbiology, University of Granada, 18003 Granada, Spain.
Metabolites. 2021 Jun 9;11(6):369. doi: 10.3390/metabo11060369.
Water deficit is one of the major constraints to crop production and food security worldwide. Some plant growth-promoting rhizobacteria (PGPR) strains are capable of increasing plant drought resistance. Knowledge about the mechanisms underlying bacteria-induced plant drought resistance is important for PGPR applications in agriculture. In this study, we show the drought stress-mitigating effects on tomato plants by the strain TG1-E1, followed by the profiling of plant transcriptomic responses to TG1-E1 and the profiling of bacterial extracellular metabolites. Comparison between the transcriptomes of drought-stressed plants with and without TG1-E1 inoculation revealed bacteria-induced transcriptome reprograming, with highlights on differentially expressed genes belonging to the functional categories including transcription factors, signal transduction, and cell wall biogenesis and organization. Mass spectrometry-based analysis identified over 40 bacterial extracellular metabolites, including several important regulators or osmoprotectant precursors for increasing plant drought resistance. These results demonstrate the importance of plant transcriptional regulation and bacterial metabolites in PGPR-induced plant drought resistance.
水分亏缺是全球作物生产和粮食安全的主要制约因素之一。一些植物促生根际细菌（PGPR）菌株能够增强植物的抗旱性。了解细菌诱导植物抗旱性的潜在机制对于PGPR在农业中的应用至关重要。在本研究中，我们展示了TG1-E1菌株对番茄植株的干旱胁迫缓解作用，随后分析了植物对TG1-E1的转录组反应以及细菌细胞外代谢产物。对接种和未接种TG1-E1的干旱胁迫植物的转录组进行比较，揭示了细菌诱导的转录组重编程，重点是属于转录因子、信号转导以及细胞壁生物合成和组织等功能类别的差异表达基因。基于质谱的分析鉴定出40多种细菌细胞外代谢产物，包括几种增强植物抗旱性的重要调节剂或渗透保护剂前体。这些结果证明了植物转录调控和细菌代谢产物在PGPR诱导的植物抗旱性中的重要性。
