Department of Dental Materials and Prosthodontics, São Paulo State University (Unesp), School of Dentistry, Araraquara, São Paulo, Brazil.
Biofouling. 2021 May;37(5):572-589. doi: 10.1080/08927014.2021.1941908. Epub 2021 Jul 1.
Understanding the interaction between oral keratinocytes (NOK-si) and is fundamental for the development of prevention strategies and new therapies for oral candidiasis. This study evaluated the dynamics and metabolic profile of these cells growing in co-culture by means of cell metabolism, number of CFU ml, and production of enzymes, cytokines, and metabolites. The data were analyzed by ANOVAs and tests (α = 0.05). In co-cultures, there were significant decreases in the cell metabolism of NOK-si and and increases in the CFU ml values of biofilm. There were also significant increases in the production of cytokines by NOK-si and proteinase by biofilm after their interaction. The metabolic balance of the main metabolites, amino acids, and extracellular and intracellular metabolites was shifted in favor of the co-cultures, while aromatic alcohols were secreted in higher amounts by the biofilm of . It was concluded that the interaction of cells in co-culture influenced their dynamics over time.
了解口腔角质细胞（NOK-si）与之间的相互作用对于开发口腔念珠菌病的预防策略和新疗法至关重要。本研究通过细胞代谢、CFU ml 数量和酶、细胞因子和代谢物的产生来评估这些细胞在共培养中的动态和代谢特征。数据分析采用方差分析和检验（α=0.05）。在共培养中，NOK-si 和 的细胞代谢显著降低，生物膜的 CFU ml 值增加。NOK-si 产生细胞因子和生物膜的蛋白酶活性增加。主要代谢物、氨基酸以及细胞外和细胞内代谢物的代谢平衡向共培养倾斜，而芳香醇则由生物膜分泌更多。结论是，共培养中细胞的相互作用会影响它们随时间的动态变化。
