Dynamics and metabolic profile of oral keratinocytes (NOK-si) and after interaction in co-culture.

Understanding the interaction between oral keratinocytes (NOK-si) and is fundamental for the development of prevention strategies and new therapies for oral candidiasis. This study evaluated the dynamics and metabolic profile of these cells growing in co-culture by means of cell metabolism, number of CFU ml, and production of enzymes, cytokines, and metabolites. The data were analyzed by ANOVAs and tests (α = 0.05). In co-cultures, there were significant decreases in the cell metabolism of NOK-si and and increases in the CFU ml values of biofilm. There were also significant increases in the production of cytokines by NOK-si and proteinase by biofilm after their interaction. The metabolic balance of the main metabolites, amino acids, and extracellular and intracellular metabolites was shifted in favor of the co-cultures, while aromatic alcohols were secreted in higher amounts by the biofilm of . It was concluded that the interaction of cells in co-culture influenced their dynamics over time.