Buijs R M, Swaab D F, Dogterom J, van Leeuwen F W
Cell Tissue Res. 1978 Jan 31;186(3):423-33. doi: 10.1007/BF00224932.
Perfusion of rat brain followed by immersion fixation with 2.5% glutaraldehyde-1% paraformaldehyde, purification of the first antisera and application of the unlabelled antibody enzyme method were used to specifically identify vasopressin and oxytocin containing cells and fibres. The conventional sites of production of these hormones were confirmed as follows: supraoptic and paraventricular nuclei, suprachiasmatic nucleus (only vasopressin), and other cells and cell groups of the hypothalamus. Fibres from the suprachiasmatic nucleus spread out in various direction, and probably project to the nucleus praeopticus periventricularis, organum vasculosum laminae terminalis and in the direction of the supraoptic nucleus. Oxytocin and vasopressin containing pathways could be traced from the paraventricular nucleus to the lateral ventricle, the stria terminalis and the stria medullaris. Some of the oxytocin and vasopressin containing tracts appear to continue onto ther septum. The possible importance of these morphological findings for the behavioural effects of vasopressin and oxytocin is discussed.
对大鼠脑进行灌注，随后用2.5%戊二醛-1%多聚甲醛进行浸入固定，纯化第一抗体并应用非标记抗体酶法，以特异性鉴定含血管加压素和催产素的细胞及纤维。这些激素的传统产生部位得到如下证实：视上核和室旁核、视交叉上核（仅含血管加压素）以及下丘脑的其他细胞和细胞群。来自视交叉上核的纤维向各个方向散开，可能投射到室周视前核、终板血管器以及视上核方向。含催产素和血管加压素的通路可从室旁核追踪至侧脑室、终纹和髓纹。部分含催产素和血管加压素的纤维束似乎延续至隔区。本文讨论了这些形态学发现对于血管加压素和催产素行为效应的可能重要性。
