Arrer E, Oberascher G, Gibitz H J
Central Laboratory, Salzburg General Hospital, Austria.
Acta Otolaryngol. 1988 Jul-Aug;106(1-2):117-23. doi: 10.3109/00016488809107378.
The large differences in the protein content of perilymph and serum as well as the perilymph volume limit of 10-15 microliter cause serious problems when collecting samples of uncontaminated perilymph in order to analyse its protein composition. In 8 out of 24 extremely carefully taken samples of perilymph removed during autopsy, we were able, by keeping to certain selection criteria, to carry out electrophoretic analyses which were suitable for reproduction and comparison. Comparison of human perilymph, CSF and serum, using SDS-Page and Western blotting, gave the expected agreement which had been suggested by tests on guinea pigs. However, a closer examination of individual proteins pointed to some relatively large differences in quantity and quality.
内淋巴液和血清中蛋白质含量的巨大差异以及10 - 15微升的内淋巴液体积限制，在采集未受污染的内淋巴液样本以分析其蛋白质组成时会引发严重问题。在尸检过程中极其小心采集的24份内淋巴液样本中，有8份样本，通过遵循特定的选择标准，我们得以进行适合重复和比较的电泳分析。使用SDS - 聚丙烯酰胺凝胶电泳（SDS - Page）和蛋白质免疫印迹法（Western blotting）对人类内淋巴液、脑脊液（CSF）和血清进行比较，得到了与豚鼠试验所提示的预期一致结果。然而，对个别蛋白质的进一步检查表明，在数量和质量上存在一些相对较大的差异。
