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[脑脊液经蜗水管流动时外淋巴替代的蛋白质研究]

[Protein study on perilymph susbstitution during cerebrospinal fluid flow through cochlear aqueduct].

作者信息

Kleinschmidt E G, Vick U

出版信息

Acta Otolaryngol. 1976 Jul-Aug;82(1-2):99-105.

PMID:1085091
Abstract

Total protein contents in the perilymphy of Scala vestibuli and Scala tympani as well as in the cerobrospinal fluid (CSF) of guinea-pigs were determined, by which specimens were taken under the following various conditions: With or without subarachnoidal puncture before perilymph collection, by varying the amount of perilymph taken from Scala tympani, by fractionating collection and under post mortem condition. The results suggest that under physiological conditions the CSF also flows through the cochleae aqueduct and the protein concentration in the Scala tympani decreases especially in the basal winding. Because the protein content in CSF is inaverage four times lower than in fluids of the inner ear it is of preference to consider the question of connection between the CSF and the fluids of the inner ear. In the specimens taken the blood contamination was estimated on the basisof erythrocytes in the connection capillaries under microscope. The protein content in the perilymph of Scala vestibuli was found significantly higher in relation to perilymph of Scala tympani.

摘要

测定了豚鼠前庭阶和鼓阶外淋巴以及脑脊液(CSF)中的总蛋白含量,样本采集条件如下:在采集外淋巴之前是否进行蛛网膜下腔穿刺、改变从鼓阶采集的外淋巴量、分阶段采集以及在死后条件下采集。结果表明,在生理条件下,脑脊液也流经蜗水管,鼓阶中的蛋白质浓度降低,尤其是在基底回。由于脑脊液中的蛋白质含量平均比内耳液中的低四倍,因此最好考虑脑脊液与内耳液之间的连通问题。在所采集的样本中,通过显微镜观察连接毛细血管中的红细胞来估计血液污染情况。发现前庭阶外淋巴中的蛋白质含量相对于鼓阶外淋巴显著更高。

相似文献

1
[Protein study on perilymph susbstitution during cerebrospinal fluid flow through cochlear aqueduct].[脑脊液经蜗水管流动时外淋巴替代的蛋白质研究]
Acta Otolaryngol. 1976 Jul-Aug;82(1-2):99-105.
2
[The dynamic behavior of inner ear fluids].[内耳液体的动态行为]
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Effect of peroral glycerol administration on inner ear fluid electrolytes of guinea pigs.经口给予甘油对豚鼠内耳液电解质的影响。
Ann Otol Rhinol Laryngol. 1985 May-Jun;94(3):319-21.
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Perilymph composition in scala tympani of the cochlea: influence of cerebrospinal fluid.耳蜗鼓阶外淋巴的成分:脑脊液的影响
Hear Res. 1989 Nov;42(2-3):265-71. doi: 10.1016/0378-5955(89)90150-0.
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[Protein concentration in the guinea-pig perilymph].[豚鼠外淋巴中的蛋白质浓度]
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[Blockage of cochlear aqueduct for examination of perilymph (guinea pig) (author's transl)].[豚鼠耳蜗导水管阻塞用于外淋巴检查(作者译)]
Arch Otorhinolaryngol. 1979;224(3-4):257-65.
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[Oxygen partial pressure measurements in the perilymph and scala tympani in normo- and hyperbaric conditions. An animal experiment study].[正常和高压条件下外淋巴和鼓阶内氧分压的测量。一项动物实验研究]
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J Assoc Res Otolaryngol. 2015 Jun;16(3):357-71. doi: 10.1007/s10162-015-0512-1. Epub 2015 Mar 24.
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Facilitated transfer of glucose from blood into perilymph in the rat cochlea.大鼠耳蜗中葡萄糖从血液到外淋巴的易化转运。
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引用本文的文献

1
[Microflame-photometric estimation of sodium and potassium for testing the purity of inner ear fluids (author's transl)].用于检测内耳液纯度的钠和钾的微火焰光度测定法(作者译)
Arch Otorhinolaryngol. 1982;234(1):45-51. doi: 10.1007/BF00453537.
2
On sources of error in the biochemical study of perilymph (guinea-pig).关于外淋巴(豚鼠)生化研究中的误差来源
Arch Otorhinolaryngol. 1984;240(1):43-8. doi: 10.1007/BF00464343.
3
[The penetration of tympanally applied tetracycline in the inner ear (author's transl)].鼓室内应用四环素在内耳的渗透情况(作者译)
Arch Otorhinolaryngol. 1978 Jan 30;218(3-4):229-38.