Laboratory of Plant Molecular Genetics, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
School of Life Science and Technology, Tokyo Institute of Technology, Tokyo, Japan.
Nat Plants. 2021 Jul;7(7):906-913. doi: 10.1038/s41477-021-00954-6. Epub 2021 Jul 1.
Bacterial cytidine deaminase fused to the DNA binding domains of transcription activator-like effector nucleases was recently reported to transiently substitute a targeted C to a T in mitochondrial DNA of mammalian cultured cells. We applied this system to targeted base editing in the Arabidopsis thaliana plastid genome. The targeted Cs were homoplasmically substituted to Ts in some plantlets of the T generation and the mutations were inherited by their offspring independently of their nuclear-introduced vectors.
细菌胞苷脱氨酶与转录激活因子样效应核酸酶的 DNA 结合域融合，最近被报道可在哺乳动物培养细胞的线粒体 DNA 中瞬时将靶标 C 替换为 T。我们将该系统应用于拟南芥质体基因组的靶向碱基编辑。在 T 代的一些植株中，靶标 C 被同型替换为 T，并且突变独立于其核引入载体遗传给后代。
