College of Health and Life Sciences, Hamad Bin Khalifa University (HBKU), Doha, Qatar.
Qatar Metabolic Institute, Hamad Medical Corporation, Doha, Qatar.
Front Endocrinol (Lausanne). 2021 Jun 15;12:638727. doi: 10.3389/fendo.2021.638727. eCollection 2021.
Polycystic ovary syndrome (PCOS) is a complex syndrome with clinical features of an endocrine/metabolic disorder. Various metabolites show significant association with PCOS; however, studies comparing the metabolic profile of pregnant women with and without PCOS are lacking. In this study, metabolomics analysis of blood samples collected from PCOS women and age and BMI matched controls in the second trimester of pregnancy was performed to identify metabolic differences between the two groups and determine their association with pregnancy outcome.
Sixteen PCOS and fifty-two healthy women in their second trimester underwent targeted metabolomics of plasma samples using tandem mass spectrometry with the Biocrates MxP Quant 500 Kit. Linear regression models were used to identify the metabolic alterations associated with PCOS, followed by enrichment and Receiver Operating Characteristic (ROC) analyses to determine the best indicators of pregnancy outcomes.
PCOS women had lower birth weight babies compared to healthy controls. As a group, systolic blood pressure (SBP) at both second trimester and at delivery negatively correlated with birth weight. Regression models indicated significant increases in the triglycerides C20:4_C34:3 and C18:2_C38:6 in the PCOS group [false discovery rate (FDR) <0.05]. Enrichment analysis revealed significant elevations in triglycerides containing arachidonic acid, linoleic acid and palmitic acid in the PCOS group. A number of indicators of baby birth weight were identified including SBP at delivery, hexosylceramide (d18:2/24:0), ceramide (d18.0/24.1) and serine, with an AUC for all predictors combined for low birth weight (≤2500grams) of 0.88 (95%CI: 0.75-1.005, p<0.001).
PCOS pregnancies resulted in babies with a lower birth weight, marked by a unique metabolic signature that was enriched with specific triglycerides and unsaturated fatty acids. The functional significance of these associations needs further investigation.
多囊卵巢综合征（PCOS）是一种具有内分泌/代谢紊乱临床特征的复杂综合征。各种代谢物与 PCOS 有显著关联；然而，比较妊娠妇女和非 PCOS 妇女代谢特征的研究还很少。在这项研究中，对妊娠中期 PCOS 妇女和年龄、BMI 匹配对照组的血液样本进行了代谢组学分析，以确定两组之间的代谢差异，并确定其与妊娠结局的关系。
16 名 PCOS 妇女和 52 名健康妇女在妊娠中期接受了基于串联质谱的靶向代谢组学血浆样本分析，使用 Biocrates MxP Quant 500 试剂盒。使用线性回归模型来确定与 PCOS 相关的代谢改变，然后进行富集和接收者操作特征（ROC）分析，以确定妊娠结局的最佳指标。
与健康对照组相比，PCOS 妇女的婴儿出生体重较低。作为一个整体，妊娠中期和分娩时的收缩压（SBP）与出生体重呈负相关。回归模型表明，PCOS 组中的甘油三酯 C20:4_C34:3 和 C18:2_C38:6 显著增加[假发现率（FDR）<0.05]。富集分析显示，PCOS 组中花生四烯酸、亚油酸和棕榈酸含量较高的甘油三酯显著升高。确定了一些与婴儿出生体重相关的指标，包括分娩时的 SBP、己糖神经酰胺（d18:2/24:0）、神经酰胺（d18.0/24.1）和丝氨酸，所有预测指标联合预测低出生体重（≤2500 克）的 AUC 为 0.88（95%CI：0.75-1.005，p<0.001）。
PCOS 妊娠导致婴儿出生体重较低，其特征是存在独特的代谢特征，富含特定的甘油三酯和不饱和脂肪酸。这些关联的功能意义需要进一步研究。
