Chen Mo-Lian, An Xin-Li, Yang Kai, Zhu Yong-Guan
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, Fujian, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Ying Yong Sheng Tai Xue Bao. 2021 Jun;32(6):2267-2274. doi: 10.13287/j.1001-9332.202106.031.
The spread of antibiotic resistance in soil is a global threat to public health and food safety, challenging the prevention and treatment of human infectious disease. The horizontal transfer of ARGs mediated by bacteriophages (phages) is an important pathway for the spread of antibiotic resistance genes (ARGs). However, the knowledge on the contribution of phages to ARGs transmission in soil is elusive. Here, we reviewed the distribution characteristics of phages in soil and its driving factors. We summarized the main methods for purification and enrichment of soil phage, reviewed recent achievements in the mechanism of phage-mediated horizontal transfer of ARGs in soil and proposed some outstanding questions. This review would contribute to understanding the important ecological role of phages in driving the horizontal transfer of ARGs, and provide a basis for developing management strategies to mitigate ARGs pollution.
抗生素耐药性在土壤中的传播是对公共卫生和食品安全的全球性威胁，对人类传染病的预防和治疗构成挑战。由噬菌体介导的抗生素耐药基因（ARGs）水平转移是抗生素耐药基因传播的重要途径。然而，关于噬菌体对土壤中ARGs传播的贡献的认识尚不清楚。在此，我们综述了土壤中噬菌体的分布特征及其驱动因素。我们总结了土壤噬菌体纯化和富集的主要方法，回顾了噬菌体介导的ARGs在土壤中水平转移机制的最新研究成果，并提出了一些突出问题。本综述将有助于理解噬菌体在驱动ARGs水平转移中的重要生态作用，并为制定减轻ARGs污染的管理策略提供依据。
