Han Runchuan, Ban Yanna, Zhen Qianna, Zhou Ying, Zhang Xiaoqing, Ding Min
Key Laboratory of Clinical Laboratory Dignostics(Ministry of Education of China), School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
Reproductive Health and Infertility Department the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Se Pu. 2019 Dec 8;37(12):1337-1342. doi: 10.3724/SP.J.1123.2019.05039.
A new method was established for the determination of indoles (indole (IND), 3-methylindole (3-MI), indolyl-3-acetic acid (IAA) and indolyl-3-propionic acid (IPA)) in plasma by high performance liquid chromatography-fluorescence detection (HPLC-FLD). The analytes were separated simultaneously on a Shim-pack VP-ODS column (150 mm×4.6 mm, 4.6 μm) using 15 mmol/L sodium dihydrogen phosphate solution and methanol (48:52, v/v) as the mobile phases. The column temperature was 30 ℃, and the flow rate was 0.8 mL/min. The calibration curves of IND, 3-MI, IPA and IAA showed good linear relationships. The intra-day and inter-day relative standard deviations (RSDs) for the analytes were both less than 6.31%. The average recoveries of the analytes in plasma were 97.5%-107.0%. The method was successfully applied to the analysis of indoles in the plasma of healthy women of reproductive age (=25) as controls and patients with polycystic ovarian syndrome (=61). The results showed that the concentrations of indoles in the plasma were significantly different between the two groups, and IND was found to be a risk factor and a potential diagnostic biomarker for polycystic ovarian syndrome (PCOS). The method is simple, sensitive and suitable for use in clinical testing and laboratory research.
