Deng Linlin, Zhen Qianna, Gao Jieying, Jin Mingchao, Ding Min, Xu Biao
Key Laboratory of Clinical Laboratory Diagnostics(Ministry of Education of China), College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
Department of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Se Pu. 2017 Jul 8;35(7):735-740. doi: 10.3724/SP.J.1123.2017.03025.
A high performance liquid chromatographic (HPLC) method with fluorescence detection (FD) was established to simultaneously determine plasma indole and skatole. Plasma samples were pretreated by liquid-liquid extraction. Chromatographic separation was accomplished on a Shim-Pack VP-ODS column (150 mm×4. 6 mm, 4.6 μ m) using an isocratic mixture of 15 mmol/L sodium dihydrogen phosphate solution and methanol (40:60, v/v). The fluorescence excitation and emission wavelengths were 274 nm and 340 nm, respectively. The linear ranges were 2.22-88.89 μ g/L for indole and 1.11-44.44 μ g/L for skatole. The detection limits were 0.11 μ g/L and 0.06 μ g/L for indole and skatole, respectively. The recoveries were in the range of 95.5%-112.3% with the relative standard deviations less than 6.8%. The method was successfully applied to the analysis of plasma from healthy pregnant women (=46) and pregnant women with hepatitis B virus (HBV) infection (=29). The results showed that plasma indole and skatole levels were significantly different between two groups. In pregnant women with HBV infection, the concentrations of indolic compounds were positively associated with transaminase levels.
建立了一种带荧光检测（FD）的高效液相色谱（HPLC）方法，用于同时测定血浆中的吲哚和粪臭素。血浆样品采用液液萃取法进行预处理。色谱分离在Shim-Pack VP-ODS柱（150 mm×4.6 mm，4.6μm）上进行，使用15 mmol/L磷酸二氢钠溶液和甲醇的等度混合物（40:60，v/v）。荧光激发波长和发射波长分别为274 nm和340 nm。吲哚的线性范围为2.22 - 88.89μg/L，粪臭素的线性范围为1.11 - 44.44μg/L。吲哚和粪臭素的检测限分别为0.11μg/L和0.06μg/L。回收率在95.5% - 112.3%范围内，相对标准偏差小于6.8%。该方法成功应用于46名健康孕妇和29名乙型肝炎病毒（HBV）感染孕妇的血浆分析。结果表明，两组之间血浆吲哚和粪臭素水平存在显著差异。在HBV感染孕妇中，吲哚类化合物的浓度与转氨酶水平呈正相关。
