State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, PR China; Fujian Key Laboratory of Genetics and Breeding of Marine Organisms, Xiamen University, Xiamen 361102, China.
State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, PR China.
Sci Total Environ. 2021 Nov 10;794:148698. doi: 10.1016/j.scitotenv.2021.148698. Epub 2021 Jun 25.
Due to anthropogenic activities that have increased global climate change and nutrient discharges, severe hypoxic events have frequently occurred in coastal waters in recent years. Relying on coastal waters, the aquaculture area has suffered ecological and economic losses caused by hypoxia, especially in summer. In this study, to investigate the stress resistance of the Pacific abalone Haliotis discus hannai (DD) and the hybrid H. discus hannai ♀ × H. fulgens ♂ (DF), a combination of physiological, biochemical, and metabolomic methods were used to compare the metabolic responses of these two abalones to acute hypoxia (0.5 mg O/L, 12 h) and reoxygenation (6.6 mg O/L, 10-20 h). Hemolymph characteristics and aerobic/anaerobic respiratory capacity changed significantly under hypoxia or reoxygenation conditions, and they were regulated in different trends in two abalones. The contents of hepatopancreas glycogen in two abalones reached the trough after 10 h recovery, implying that short-term hypoxia leads to a long-lasting (several hours) imprint on the energy storage of abalone. In response to dissolved oxygen fluctuation, metabolic profiles of two abalones changed in distinct ways both in the hypoxia group or the reoxygenation group. The conversion of carbohydrate metabolism and amino acid metabolism indicated that hypoxia prompts abalone to change the way of energy metabolism, which may also reflect the difference in the energy utilization of DD and DF abalones. In addition, 3 metabolites (L-glutamate, 2-hydroxy-butanoic acid, and 2-methyl-3-hydroxybutyric acid) as potential biomarkers for hypoxia and reoxygenation response in abalone were determined by operating characteristic analysis (ROC). Overall, this study provides information towards understanding the damage caused by frequent hypoxic events and implies the metabolic shifts that occur under hypoxia and reoxygenation conditions in DD and DF abalones.
由于人类活动导致全球气候变化和营养物质排放增加，近年来沿海海域频繁发生严重缺氧事件。依靠沿海海域，水产养殖区遭受了缺氧造成的生态和经济损失，尤其是在夏季。在这项研究中，为了研究太平洋鲍 Haliotis discus hannai（DD）和杂交 H. discus hannai♀×H. fulgens♂（DF）的抗应激能力，采用生理、生化和代谢组学方法相结合，比较了这两种鲍对急性缺氧（0.5 mg O/L，12 h）和再氧合（6.6 mg O/L，10-20 h）的代谢反应。在缺氧或再氧合条件下，血液特征和有氧/无氧呼吸能力发生了显著变化，并且在两种鲍中以不同的趋势进行调节。两种鲍的肝胰腺糖原含量在 10 h 恢复后达到低谷，这表明短期缺氧会对鲍鱼的能量储存产生持久（数小时）的影响。针对溶解氧波动，两种鲍的代谢谱在缺氧组或再氧合组中以不同的方式发生变化。碳水化合物代谢和氨基酸代谢的转换表明，缺氧促使鲍鱼改变能量代谢方式，这也可能反映了 DD 和 DF 鲍鱼在能量利用方面的差异。此外，通过操作特征分析（ROC）确定了 3 种代谢物（L-谷氨酸、2-羟基丁酸和 2-甲基-3-羟基丁酸）作为鲍对缺氧和再氧合反应的潜在生物标志物。总体而言，本研究提供了有关频繁缺氧事件造成的损害的信息，并暗示了 DD 和 DF 鲍在缺氧和再氧合条件下发生的代谢变化。
