Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu, China.
Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu, China.
Biochem Biophys Res Commun. 2021 Sep 3;568:103-109. doi: 10.1016/j.bbrc.2021.06.070. Epub 2021 Jun 30.
At present, stem cell transplantation has a significant therapeutic effect on spinal cord injury (SCI), however, it is still challenging for the seed cells selection. In this study, in order to explore cells with wide neural repair potentials, we selected the pluripotent stem cells multilineage-differentiating stress-enduring (Muse) cells, inducing the in vitro differentiation of human Muse cells into neural precursor cells (Muse-NPCs) by applying neural induction medium. Here, we found induced Muse-NPCs expressed neural stem cell markers Nestin and NCAM, capable of differentiating into three types of neural cells (neuron, astrocyte and oligodendrocyte), and have certain biological functions. When Muse-NPCs were transplanted into rats suffering from T10 SCI, motor function was improved. These results provide an insight for application of Muse-NPCs in cell therapy or tissue engineering for the repair of SCI in future.
目前，干细胞移植对脊髓损伤（SCI）具有显著的治疗效果，但种子细胞的选择仍然具有挑战性。在这项研究中，为了探索具有广泛神经修复潜力的细胞，我们选择了多能干细胞多系分化应激耐受（Muse）细胞，通过应用神经诱导培养基将人 Muse 细胞在体外诱导分化为神经前体细胞（Muse-NPC）。在这里，我们发现诱导的 Muse-NPC 表达神经干细胞标志物巢蛋白和 NCAM，能够分化为三种类型的神经细胞（神经元、星形胶质细胞和少突胶质细胞），并具有一定的生物学功能。当 Muse-NPC 被移植到 T10 SCI 大鼠中时，运动功能得到了改善。这些结果为 Muse-NPC 在未来用于 SCI 细胞治疗或组织工程修复的应用提供了新的思路。
