National Defence Ministry, 5th Main Maintenance Factory Directorate, 06797 Ankara, Turkey.
Department of Chemical Engineering, Hacettepe University, 06800 Ankara, Turkey.
J Biosci Bioeng. 2021 Sep;132(3):234-240. doi: 10.1016/j.jbiosc.2021.05.007. Epub 2021 Jun 30.
This study aimed to evaluate the effects of by-product molasses and waste orange peels (WOP) as the nutrient medium on lipase production with Rhizopus arrhizus NRRL 2286 and investigate the inductive effect of waste cooking oil (WCO) on activity. The results showed that the highest specific growth rate and the maximum biomass concentration were obtained 0.424 h and 3.83 g dry weight/L for 70 g/L WOP, respectively. Additionally, the highest intracellular and extracellular lipase activities were attained in 50 g/L WOP as 400 U/g and 13.2 U/mL, correspondingly. Next, the inductive effects of WCO and olive oil were tested in lipase production. Intracellular lipase activity enhanced and reached a maximum of 520 U/g in the presence of 10 g/L WCO with an increase of 30% compared to the one without WCO. However, both inducers caused a diminish in extracellular lipase hydrolysis activity. Overall, this study reveals that the lipase production of R. arrhizus can be accomplished using WOP and WCO with the optimum conditions.
本研究旨在评估副产物糖蜜和废橙皮（WOP）作为营养介质对 Rhizopus arrhizus NRRL 2286 脂肪酶生产的影响，并研究废食用油（WCO）对其活性的诱导作用。结果表明，当 WOP 为 70g/L 时，获得了最高的比生长速率（0.424h）和最大生物量浓度（3.83g 干重/L）。此外，在 50g/L WOP 中获得了最高的细胞内和细胞外脂肪酶活性，分别为 400U/g 和 13.2U/mL。接下来，测试了 WCO 和橄榄油对脂肪酶生产的诱导作用。在 10g/L WCO 的存在下，细胞内脂肪酶活性增强，达到 520U/g，比没有 WCO 的情况下提高了 30%。然而，两种诱导剂都导致细胞外脂肪酶水解活性下降。总的来说，本研究表明，使用 WOP 和 WCO 可以在最佳条件下完成 R. arrhizus 的脂肪酶生产。
