D'Annibale Alessandro, Sermanni Giovanni Giovannozzi, Federici Federico, Petruccioli Maurizio
Dipartimento di Agrobiologia e Agrochimica, University of Tuscia, Viterbo, Italy.
Bioresour Technol. 2006 Oct;97(15):1828-33. doi: 10.1016/j.biortech.2005.09.001. Epub 2005 Oct 19.
The present study investigated the valorization of olive-mill wastewater (OMW) by its use as a possible growth medium for the microbial production of extra-cellular lipase. To this end, strains of Geotrichum candidum (NRRL Y-552 and Y-553), Rhizopus arrhizus (NRRL 2286 and ISRIM 383), Rhizopus oryzae (NRRL 6431), Aspergillus oryzae (NRRL 1988 and 495), Aspergillus niger (NRRL 334), Candida cylindracea (NRRL Y-17506) and Penicillium citrinum (NRRL 1841 and 3754, ISRIM 118) were screened. All strains were able to grow on the undiluted OMW, producing extra-cellular lipase activity. C. cylindracea NRRL Y-17506 showed the highest lipase activity on all the typologies of OMW used. Its lipase production on OMW was markedly affected by the type of nitrogen source and was induced by the addition of olive oil. The highest activity (9.23 IU ml(-1)) of the yeast was obtained on OMW supplemented with NH(4)Cl (2.4 g l(-1)) and olive oil (3.0 g l(-1)).
本研究通过将橄榄油厂废水（OMW）用作微生物生产胞外脂肪酶的可能生长培养基，对其价值进行了研究。为此，筛选了白地霉（NRRL Y - 552和Y - 553）、少根根霉（NRRL 2286和ISRIM 383）、米根霉（NRRL 6431）、米曲霉（NRRL 1988和495）、黑曲霉（NRRL 334）、柱形假丝酵母（NRRL Y - 17506）和桔青霉（NRRL 1841和3754，ISRIM 118）的菌株。所有菌株都能在未稀释的OMW上生长，并产生胞外脂肪酶活性。柱形假丝酵母NRRL Y - 17506在所用的所有类型的OMW上均表现出最高的脂肪酶活性。其在OMW上的脂肪酶产生受到氮源类型的显著影响，并因添加橄榄油而诱导。在添加了NH₄Cl（2.4 g l⁻¹）和橄榄油（3.0 g l⁻¹）的OMW上获得了该酵母的最高活性（9.23 IU ml⁻¹）。
