College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Int J Biol Macromol. 2021 Aug 31;185:782-791. doi: 10.1016/j.ijbiomac.2021.06.190. Epub 2021 Jun 30.
In this work, a novel DHA-loaded nanoparticle with PLGA and chitosan (PCSDNP) was successfully prepared. The structure of PCSDNP and DHA-loaded PLGA nanoparticles was measured by transmission electron microscope, scanning electron microscope, and differential scanning calorimeter. The interaction strength between DHA, PLGA, and chitosan was evaluated through Fourier transform infrared spectroscopy. The curves of controlled DHA release and stabilities for different environmental factors of two NPs were evaluated. Importantly, two NPs were almost regularly spherical and the interactions were hydrogen bonds and electrostatic interactions between PLGA and chitosan. These NPs had a good encapsulation rate (80.45%) and high-water solubility than the free DHA molecule. In simulated gastrointestinal fluid, two NPs showed a controlled-release pattern. Overall, PCSDNP had better stability and controlled-release effect with the synergy between CS and PLGA under the conditions of pH (2- 7), ionic strength (0- 500 mM), storage time (0- 42 d), and temperature (30- 80 °C).
在这项工作中，成功制备了一种新型的载二十二碳六烯酸（DHA）的纳米粒子，其载体为聚乳酸-羟基乙酸共聚物（PLGA）和壳聚糖（PCSDNP）。通过透射电子显微镜、扫描电子显微镜和差示扫描量热仪测量了 PCSDNP 和载 DHA 的 PLGA 纳米粒子的结构。通过傅里叶变换红外光谱评估了 DHA、PLGA 和壳聚糖之间的相互作用强度。评估了两种纳米粒子在不同环境因素下控制 DHA 释放和稳定性的曲线。重要的是，两种纳米粒子几乎都是规则的球形，相互作用是 PLGA 和壳聚糖之间的氢键和静电相互作用。这些纳米粒子具有良好的包封率（80.45%）和高于游离 DHA 分子的高水溶性。在模拟胃肠道液中，两种纳米粒子均表现出控制释放模式。总体而言，在 pH（2-7）、离子强度（0-500 mM）、储存时间（0-42 天）和温度（30-80°C）条件下，CS 和 PLGA 的协同作用使 PCSDNP 具有更好的稳定性和控制释放效果。
