College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
Food Chem. 2021 Oct 30;360:129957. doi: 10.1016/j.foodchem.2021.129957. Epub 2021 Apr 28.
This study focused on the feasibility of delivering docosahexaenoic acid (DHA) via zein/ Poly(lactic-co-glycolic) acid stabilized nanoparticles (ZPDNPs). ZPDNPs with good encapsulation efficiency, water solubility, and chemical instability were prepared by the antisolvent precipitation method. The structure and interaction force of ZPDNPs was evaluated using transmission electron microscopy, circular dichroism, X-ray powder diffraction, and FTIR. The stabilities of ZPDNPs for heating, pH, NaCl aqueous solution were also investigated. The results showed that ZPDNPs can improve the water solubility (750-fold), salt tolerance, and heat-resistant processing stability of DHA. The ZPDNPs had high encapsulation efficiency (84.6%) and stability after 35 days of storage. The simulated digestion of the gastrointestinal tract can change particle size, interface potential, and aggregation state of different DHA nanoparticles. The bioavailability of ZPDNPs was 4.2 ~ 5.6-folds higher than free DHA. Overall, a useful method for ZPDNPs formulation was developed for the delivery of DHA.
本研究聚焦于通过玉米醇溶蛋白/聚（乳酸-共-乙醇酸）稳定的纳米颗粒（ZPDNPs）递送二十二碳六烯酸（DHA）的可行性。通过抗溶剂沉淀法制备了具有良好包封效率、水溶性和化学不稳定性的 ZPDNPs。使用透射电子显微镜、圆二色性、X 射线粉末衍射和傅里叶变换红外光谱评估了 ZPDNPs 的结构和相互作用力。还研究了 ZPDNPs 在加热、pH 值和 NaCl 水溶液中的稳定性。结果表明，ZPDNPs 可以提高 DHA 的水溶性（750 倍）、耐盐性和耐热加工稳定性。ZPDNPs 具有高包封效率（84.6%）和储存 35 天后的稳定性。胃肠道模拟消化可以改变不同 DHA 纳米颗粒的粒径、界面电位和聚集状态。ZPDNPs 的生物利用度比游离 DHA 高 4.2 至 5.6 倍。总的来说，为 DHA 的递送开发了一种有用的 ZPDNPs 配方方法。
