Federal University of Santa Maria (UFSM), Laboratory of Mycotoxicological Analyses (LAMIC), Santa Maria (SM), Rio Grande do Sul (RS), Brazil.
Pegasus Science, SM, RS, Brazil.
Toxicon. 2021 Sep;200:102-109. doi: 10.1016/j.toxicon.2021.06.016. Epub 2021 Jul 1.
The impact of deoxynivalenol (DON) upon intestinal tissue of broilers was assessed by using jejunal explants in Ussing chambers and analyzing histopathological and immunohistochemical parameters; this system was also applied to evaluate the efficacy of an antimycotoxins additive (AMA). The explants were subjected to the following treatments within each experiment for 120 min: Experiment 1) T1 (control) - buffer solution, and T2 - 10 mg/L DON; and Experiment 2) T1 (control) - buffer solution, T2 - 10 mg/L DON, T3 - AMA (0.5%), and T4 - 10 mg/L DON + 0.5% AMA. In Experiment 1, DON triggered a reduction in the size of enterocytes as well as of their nuclei, an increase in cytoplasmic vacuolization and apical denudation of villi. Apoptotic cells count was also greater in DON-exposed explants. In Experiment 2, the AMA mitigated DON harmful effects; cytoplasmic vacuolization of enterocytes was reduced and the size of their nuclei was preserved. The additive also promoted a partial decrease in microvillus integrity, in size of enterocytes and in apoptotic cells count. The tested ex vivo model demonstrated the impact of DON upon the intestine as well as the efficacy of the AMA against its damaging effects.
采用 Ussing 室中的空肠组织进行离体实验，分析组织病理学和免疫组织化学参数，评估脱氧雪腐镰刀菌烯醇（DON）对肉鸡肠道组织的影响；该系统还用于评估一种霉菌毒素吸附剂（AMA）的功效。在每个实验中，将离体组织用以下处理方式孵育 120 分钟：实验 1）T1（对照）-缓冲液，T2-10mg/L DON；实验 2）T1（对照）-缓冲液，T2-10mg/L DON，T3-0.5% AMA，T4-10mg/L DON+0.5% AMA。在实验 1 中，DON 可导致肠细胞及其细胞核变小，细胞质空泡化和绒毛顶端脱屑增加。暴露于 DON 的离体组织中凋亡细胞的数量也更多。在实验 2 中，AMA 减轻了 DON 的有害影响；肠细胞的细胞质空泡化减少，细胞核大小得以保留。该添加剂还促进了微绒毛完整性、肠细胞大小和凋亡细胞计数的部分减少。该体外实验模型证明了 DON 对肠道的影响以及 AMA 对其有害影响的功效。
