Cortez Barbara Ribeiro de Souza, Guedes Roberto Maurício Carvalho
Department of Veterinary Clinic and Surgery, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Department of Large Animal Clinical Sciences, University of Saskatchewan, Saskatoon, Canada.
Vet World. 2023 Feb;16(2):347-356. doi: 10.14202/vetworld.2023.347-356. Epub 2023 Feb 21.
Different techniques have been reported in studies of intestinal organ culture (IVOC). A robust compilation of all available methods is lacking in the literature, making it difficult to choose a method that corresponds to the study's demands. In this review, readers can assess the most available methods, allowing them to evaluate which is more suitable for their purposes and requirements. A simplified view of culturing intestinal explants is presented, highlighting the approachability of IVOC. Relevant findings from diverse veterinarian studies, where explants played a major role, as well as the technique used in each, are described to illustrate its applications. Finally, the strengths and limitations of the innovative intestinal IVOC methods are discussed. This review provides a collection of methods for intestinal explant culture and their possible applications in veterinary research. In this way, it aims to broaden access to IVOC techniques and aid decision-making regarding the best suited for a study's purposes.
在肠道器官培养（IVOC）研究中已报道了不同的技术。文献中缺乏对所有可用方法的全面汇编，这使得难以选择符合研究需求的方法。在本综述中，读者可以评估最常用的方法，从而能够评估哪种方法更适合其目的和要求。本文呈现了培养肠道外植体的简化观点，突出了IVOC的可及性。描述了各种以肠道外植体为主的兽医研究的相关发现以及其中使用的技术，以说明其应用。最后，讨论了创新性肠道IVOC方法的优缺点。本综述提供了肠道外植体培养方法及其在兽医研究中可能应用的汇总。通过这种方式，旨在拓宽对IVOC技术的获取渠道，并有助于就最适合研究目的的方法做出决策。
