College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin 130118, China; Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun, Jilin 130118, China; Ministry of Education Laboratory of Animal Production and Quality Security, Jilin Agricultural University, Changchun, Jilin 130118, China.
Xin Li Cheng Reservoir Administration Bureau of Jilin Province, Changchun, Jilin 130022, China.
Comp Biochem Physiol C Toxicol Pharmacol. 2021 Nov;249:109121. doi: 10.1016/j.cbpc.2021.109121. Epub 2021 Jul 1.
The present study investigated the effects of dietary Astragalus Propinquus schischkin polysaccharides on growth, immune responses, antioxidants responses and inflammation-related genes expression in Channa argus. Channa argus were randomly divided into 5 groups and fed 5 levels diets of A. propinquus schischkin polysaccharides (0, 250, 500, 1000 and 2000 mg/kg) for 56 days. The results showed, dietary A. propinquus schischkin polysaccharides addition can increase the final body weight, weight gain and specific growth rate, decrease the feed conversion ratio of Channa argus. And dietary A. propinquus schischkin polysaccharides supplementation can increase the levels of serum superoxide dismutase, catalase, glutathione peroxidase, lysozyme, complement 3, complement 4, immunoglobulin M and alkaline phosphatase, decrease the levels of serum malondialdehyde, cortisol, aspartate aminotransferase and glutamic-pyruvic transaminase. Furthermore, dietary A. propinquus schischkin polysaccharides can decrease the gene expression levels of interleukin-1β, interleukin-, tumor necrosis factor-α and nuclear factor-κB, increase the gene expression levels of glucocorticoid receptor in liver, spleen, kidney, intestine. To sum up, dietary A. propinquus schischkin polysaccharides can accelerate growth, enhance immune responses and antioxidants responses, regulate inflammation-related genes expression in Channa argus and the optimum amount is 1000 mg/kg.
本研究探讨了日粮黄芪多糖对乌鳢生长、免疫应答、抗氧化应激及炎症相关基因表达的影响。将乌鳢随机分为 5 组，分别投喂 5 种不同水平（0、250、500、1000 和 2000mg/kg）的黄芪多糖日粮，56 天后进行检测。结果表明，日粮添加黄芪多糖能提高乌鳢的末重、增重率和特定生长率，降低饲料系数。同时，还能提高血清中超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、溶菌酶、补体 3、补体 4、免疫球蛋白 M 和碱性磷酸酶的活性，降低血清丙二醛、皮质醇、天门冬氨酸转氨酶和谷氨酸丙酮酸转氨酶的含量。此外，黄芪多糖还能降低肝脏、脾脏、肾脏和肠道中白细胞介素-1β、白细胞介素-1β、肿瘤坏死因子-α和核因子-κB 的基因表达水平，提高肝脏、脾脏、肾脏和肠道中糖皮质激素受体的基因表达水平。综上所述，日粮添加黄芪多糖能促进乌鳢生长，增强免疫和抗氧化应激能力，调节炎症相关基因的表达，其最适添加量为 1000mg/kg。
