Sadek Ahmed H, Asker Mohsen S, Abdelhamid Sayeda A
Environmental Engineering Program, Zewail City of Science, Technology and Innovation, 6th October City, Giza, 12578 Egypt.
Sanitary and Environmental Engineering Research Institute, Housing and Building National Research Center (HBRC), Dokki, Giza, 11511 Egypt.
Biologia (Bratisl). 2021;76(9):2785-2809. doi: 10.1007/s11756-021-00814-w. Epub 2021 Jun 28.
Nanoscale zero-valent iron particles were investigated as an antibacterial agent against two Gram-positive bacteria; NRRL B-313 (), NRC (), and two Gram-negative bacteria; NRC B-3703 (), NRC B-32 (). The characterization of synthesized nZVI particles was obtained by XRD, SEM, EDX, and TG analyses. The results demonstrated that the nZVI particles have a spherical shape, mean crystalline size of 44.43 nm, and exhibited a good chemical and thermal stability performance under different physical conditions. The bacterial suspensions were subjected to the treatment using nZVI particle suspensions with a concentration of 10 mg/mL. The minimum inhibitory concentration of nZVI particles was determined using the well diffusion assay method and found to be 15, 10, 10, and 5 mg for the following four strains; , , , and , respectively. The biological treatment results of municipal wastewater using nZVI particles revealed that the counts of total bacteria, total coliform, fecal coliform, , , and were decreased to 44.29%, 51.76%, 90.95%, 46.67%, 33.33%, and 93.89%, respectively, while the not detected in the wastewater sample. The enhanced inactivation performance of nZVI nanoparticles was mainly attributed to the reactive oxygen species (ROS) production, releasing of iron corrosion products like Fe/Fe ions, and direct friction of nZVI particles with bacterial cells membranes. In addition, the nZVI particles presented a striking disinfection behavior in comparison with other widespread disinfection technologies such as chlorination. Accordingly, the obtained results introduce the nZVI particles as a promising disinfection technology.
研究了纳米级零价铁颗粒作为抗两种革兰氏阳性菌（NRRL B - 313、NRC）和两种革兰氏阴性菌（NRC B - 3703、NRC B - 32）的抗菌剂。通过X射线衍射（XRD）、扫描电子显微镜（SEM）、能量色散X射线光谱（EDX）和热重分析（TG）对合成的纳米零价铁（nZVI）颗粒进行了表征。结果表明，nZVI颗粒呈球形，平均晶体尺寸为44.43纳米，并且在不同物理条件下表现出良好的化学和热稳定性。细菌悬液用浓度为10毫克/毫升的nZVI颗粒悬液进行处理。使用平板扩散法测定nZVI颗粒的最低抑菌浓度，发现对于以下四种菌株，最低抑菌浓度分别为15毫克、10毫克、10毫克和5毫克，对应菌株分别为、、、。使用nZVI颗粒对城市污水进行生物处理的结果表明，总细菌、总大肠菌群、粪大肠菌群、、和的数量分别降至44.29%、51.76%、90.95%、46.67%、33.33%和93.89%，而污水样品中未检测到。nZVI纳米颗粒增强的灭活性能主要归因于活性氧（ROS）的产生、铁腐蚀产物如铁离子/亚铁离子的释放以及nZVI颗粒与细菌细胞膜的直接摩擦。此外，与其他广泛使用的消毒技术如氯化相比，nZVI颗粒表现出显著的消毒行为。因此，所得结果表明nZVI颗粒是一种有前景的消毒技术。
