Campbell P B, Tolson T A
Department of Medicine, East Carolina University School of Medicine, Greenville, North Carolina 27858-4354.
J Leukoc Biol. 1988 Feb;43(2):117-24. doi: 10.1002/jlb.43.2.117.
The leukotactic responsiveness of human peripheral blood monocytes is regulated by the cell-directed inhibitor of monocyte leukotaxis, CDI-MLx. The actions of CDI-MLx on normal monocytes in vitro were abrogated by co-incubation with inhibitors of cyclooxygenase and thromboxane synthetase with indomethacin and dazmegrel (UK-38,485) being most active. The actions of CDI-MLx were mimicked by the thromboxane A2 analogue, U-46619, and by 12-HHT with half-maximal inhibition observed at 10(-10) M; PGE2 was 1000-fold less active. SQ 29,548, a thromboxane A2 receptor antagonist, blocked the effects of CDI-MLx, U-46619, and 12-HHT. Production of PGE2 and thromboxane B2 by purified monocytes was stimulated by CDI-MLx and this effect was also blocked by indomethacin, dazmegrel, and dazoxiben. These data suggest a major regulatory role for thromboxane synthetase products in human monocyte leukotaxis.
人外周血单核细胞的白细胞趋化反应性受单核细胞白细胞趋化作用的细胞定向抑制剂CDI-MLx调控。在体外，通过与环氧化酶和血栓素合成酶抑制剂共同孵育，可消除CDI-MLx对正常单核细胞的作用，其中吲哚美辛和达美格雷（UK-38,485）最为有效。血栓素A2类似物U-46619和12-羟基-5,8,10,14-二十碳四烯酸（12-HHT）可模拟CDI-MLx的作用，在10^(-10) M时观察到半数最大抑制；前列腺素E2（PGE2）的活性低1000倍。血栓素A2受体拮抗剂SQ 29,548可阻断CDI-MLx、U-46619和12-HHT的作用。纯化的单核细胞产生PGE2和血栓素B2的过程受到CDI-MLx的刺激，吲哚美辛、达美格雷和达唑氧苯也可阻断这一作用。这些数据表明血栓素合成酶产物在人单核细胞白细胞趋化中起主要调节作用。
